EXAFS studies of the isolated bovine heart Rieske [2Fe-2S]1+(1+,2+) cluster

L. Powers; H. Schagger; G. von^Jagow; J. Smith; B. Chance; T. Ohnishi

doi:10.1016/S0005-2728(89)80261-0

EXAFS studies of the isolated bovine heart Rieske [2Fe-2S]^1+(1+,2+) cluster

L. Powers
, H. Schagger
, G. von^Jagow
, J. Smith
, B. Chance
, T. Ohnishi

Electrical and Computer Engr

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Recently the involvement of one or, more likely, two nitrogen- ligands in the Rieske-type [2Fe-2S] cluster has been reported based on the chemical assay and various spectroscopic analyses, such as EPR, Mössbauer, ENDOR, and resonance Raman, of isolated Thermus thermophilus HB-8 protein by Fee and his collaborators. Similarly, the presence of at least one nitrogen ligand was shown in the mitochondrial Rieske [2Fe-2S] cluster. We have conducted EXAFS studies of the Rieske [2Fe-2S] protein isolated from the cytochrome {itbc₁ complex of bovine heart mitochondria. Standard analysis could not distinguish one or two nitrogen ligands per cluster. However, one nitrogen and three cysteine ligands per cluster was found to be, possibly, a better solution in more comprehensive analysis procedures.

Original language	English (US)
Pages (from-to)	293-298
Number of pages	6
Journal	BBA - Bioenergetics
Volume	975
Issue number	2
DOIs	https://doi.org/10.1016/S0005-2728(89)80261-0
State	Published - Jul 1989

Keywords

(Bovine heart)
Cytochrome bc
Iron-sulfur cluster
Nitrogen ligand
Rieske protein

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

Access to Document

10.1016/S0005-2728(89)80261-0

Cite this

@article{2c0ddada68a7478e8d91897a0c0d9401,

title = "EXAFS studies of the isolated bovine heart Rieske [2Fe-2S]1+(1+,2+) cluster",

abstract = "Recently the involvement of one or, more likely, two nitrogen- ligands in the Rieske-type [2Fe-2S] cluster has been reported based on the chemical assay and various spectroscopic analyses, such as EPR, M{\"o}ssbauer, ENDOR, and resonance Raman, of isolated Thermus thermophilus HB-8 protein by Fee and his collaborators. Similarly, the presence of at least one nitrogen ligand was shown in the mitochondrial Rieske [2Fe-2S] cluster. We have conducted EXAFS studies of the Rieske [2Fe-2S] protein isolated from the cytochrome \{itbc1 complex of bovine heart mitochondria. Standard analysis could not distinguish one or two nitrogen ligands per cluster. However, one nitrogen and three cysteine ligands per cluster was found to be, possibly, a better solution in more comprehensive analysis procedures.",

keywords = "(Bovine heart), Cytochrome bc, Iron-sulfur cluster, Nitrogen ligand, Rieske protein",

author = "L. Powers and H. Schagger and G. von\textasciicircum{}Jagow and J. Smith and B. Chance and T. Ohnishi",

note = "Funding Information: The authors thank Dr. Jonathan Freedman for his assistance in data collection. They also thank Dr.J. Penner-Hahn for helpful discussions and preprints on T. thermophilus Rieske iron-sulfur-nitrogen protein prior to publication. X-ray absorption data was collected at the Stanford Synchrotron Radiation laboratory, supported by the National Science Foundation, Division of Materials Research; Department of Energy, Office of Basic Energy Sciences; and the National Institutes of Health, Biotechnology Research programs, Division of Research Resources. This investigation was also supported by Deutsche Forschungsgemeinschaft to G.V.J., NSF DMB85-11160 to T.O., and Research Corporation Award No. 9371 to J.S.",

year = "1989",

month = jul,

doi = "10.1016/S0005-2728(89)80261-0",

language = "English (US)",

volume = "975",

pages = "293--298",

journal = "BBA - Bioenergetics",

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publisher = "Elsevier B.V.",

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AU - Powers, L.

AU - Schagger, H.

AU - von^Jagow, G.

AU - Smith, J.

AU - Chance, B.

AU - Ohnishi, T.

N1 - Funding Information: The authors thank Dr. Jonathan Freedman for his assistance in data collection. They also thank Dr.J. Penner-Hahn for helpful discussions and preprints on T. thermophilus Rieske iron-sulfur-nitrogen protein prior to publication. X-ray absorption data was collected at the Stanford Synchrotron Radiation laboratory, supported by the National Science Foundation, Division of Materials Research; Department of Energy, Office of Basic Energy Sciences; and the National Institutes of Health, Biotechnology Research programs, Division of Research Resources. This investigation was also supported by Deutsche Forschungsgemeinschaft to G.V.J., NSF DMB85-11160 to T.O., and Research Corporation Award No. 9371 to J.S.

PY - 1989/7

Y1 - 1989/7

N2 - Recently the involvement of one or, more likely, two nitrogen- ligands in the Rieske-type [2Fe-2S] cluster has been reported based on the chemical assay and various spectroscopic analyses, such as EPR, Mössbauer, ENDOR, and resonance Raman, of isolated Thermus thermophilus HB-8 protein by Fee and his collaborators. Similarly, the presence of at least one nitrogen ligand was shown in the mitochondrial Rieske [2Fe-2S] cluster. We have conducted EXAFS studies of the Rieske [2Fe-2S] protein isolated from the cytochrome {itbc1 complex of bovine heart mitochondria. Standard analysis could not distinguish one or two nitrogen ligands per cluster. However, one nitrogen and three cysteine ligands per cluster was found to be, possibly, a better solution in more comprehensive analysis procedures.

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KW - (Bovine heart)

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KW - Nitrogen ligand

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