TY - JOUR
T1 - EXAFS studies of the isolated bovine heart Rieske [2Fe-2S]1+(1+,2+) cluster
AU - Powers, L.
AU - Schagger, H.
AU - von^Jagow, G.
AU - Smith, J.
AU - Chance, B.
AU - Ohnishi, T.
N1 - Funding Information:
The authors thank Dr. Jonathan Freedman for his assistance in data collection. They also thank Dr.J. Penner-Hahn for helpful discussions and preprints on T. thermophilus Rieske iron-sulfur-nitrogen protein prior to publication. X-ray absorption data was collected at the Stanford Synchrotron Radiation laboratory, supported by the National Science Foundation, Division of Materials Research; Department of Energy, Office of Basic Energy Sciences; and the National Institutes of Health, Biotechnology Research programs, Division of Research Resources. This investigation was also supported by Deutsche Forschungsgemeinschaft to G.V.J., NSF DMB85-11160 to T.O., and Research Corporation Award No. 9371 to J.S.
PY - 1989/7
Y1 - 1989/7
N2 - Recently the involvement of one or, more likely, two nitrogen- ligands in the Rieske-type [2Fe-2S] cluster has been reported based on the chemical assay and various spectroscopic analyses, such as EPR, Mössbauer, ENDOR, and resonance Raman, of isolated Thermus thermophilus HB-8 protein by Fee and his collaborators. Similarly, the presence of at least one nitrogen ligand was shown in the mitochondrial Rieske [2Fe-2S] cluster. We have conducted EXAFS studies of the Rieske [2Fe-2S] protein isolated from the cytochrome {itbc1 complex of bovine heart mitochondria. Standard analysis could not distinguish one or two nitrogen ligands per cluster. However, one nitrogen and three cysteine ligands per cluster was found to be, possibly, a better solution in more comprehensive analysis procedures.
AB - Recently the involvement of one or, more likely, two nitrogen- ligands in the Rieske-type [2Fe-2S] cluster has been reported based on the chemical assay and various spectroscopic analyses, such as EPR, Mössbauer, ENDOR, and resonance Raman, of isolated Thermus thermophilus HB-8 protein by Fee and his collaborators. Similarly, the presence of at least one nitrogen ligand was shown in the mitochondrial Rieske [2Fe-2S] cluster. We have conducted EXAFS studies of the Rieske [2Fe-2S] protein isolated from the cytochrome {itbc1 complex of bovine heart mitochondria. Standard analysis could not distinguish one or two nitrogen ligands per cluster. However, one nitrogen and three cysteine ligands per cluster was found to be, possibly, a better solution in more comprehensive analysis procedures.
KW - (Bovine heart)
KW - Cytochrome bc
KW - Iron-sulfur cluster
KW - Nitrogen ligand
KW - Rieske protein
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U2 - 10.1016/S0005-2728(89)80261-0
DO - 10.1016/S0005-2728(89)80261-0
M3 - Article
C2 - 2545269
AN - SCOPUS:0024315516
VL - 975
SP - 293
EP - 298
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
SN - 0005-2728
IS - 2
ER -