Evaluation of whole blood theophylline enzyme immunochromatography assay

Michael P Habib; R. B. Schifman; B. Y. Shon; J. F. Fiastro; S. C. Campbell

doi:10.1378/chest.92.1.129

Evaluation of whole blood theophylline enzyme immunochromatography assay

Michael P Habib, R. B. Schifman, B. Y. Shon, J. F. Fiastro, S. C. Campbell

Pathology

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Abstract

A new whole blood enzyme immunochromatographic (EIC) theophylline assay was evaluated in 18 low (25 to 37 percent) and 15 high hematocrit (49 to 56 percent) samples. A good correlation was observed between EIC and fluorescence polarization methods for plasma samples (r = 0.95). However, comparison of results between EIC whole blood and plasma values demonstrates a significant proportional bias that is inversely related to the sample's hematocrit. The EIC method for whole blood samples may substantially underestimate theophylline levels in polycythemic patients with theophylline values near or above the toxic range and underestimate levels in those with anemia, if a correction is not made for the sample's hematocrit. A correction formula to approximate plasma theophylline concentrations from whole blood measurements is described.

Original language	English (US)
Pages (from-to)	129-131
Number of pages	3
Journal	CHEST
Volume	92
Issue number	1
DOIs	https://doi.org/10.1378/chest.92.1.129
State	Published - 1987

ASJC Scopus subject areas

Pulmonary and Respiratory Medicine
Critical Care and Intensive Care Medicine
Cardiology and Cardiovascular Medicine

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10.1378/chest.92.1.129

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title = "Evaluation of whole blood theophylline enzyme immunochromatography assay",

abstract = "A new whole blood enzyme immunochromatographic (EIC) theophylline assay was evaluated in 18 low (25 to 37 percent) and 15 high hematocrit (49 to 56 percent) samples. A good correlation was observed between EIC and fluorescence polarization methods for plasma samples (r = 0.95). However, comparison of results between EIC whole blood and plasma values demonstrates a significant proportional bias that is inversely related to the sample's hematocrit. The EIC method for whole blood samples may substantially underestimate theophylline levels in polycythemic patients with theophylline values near or above the toxic range and underestimate levels in those with anemia, if a correction is not made for the sample's hematocrit. A correction formula to approximate plasma theophylline concentrations from whole blood measurements is described.",

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T1 - Evaluation of whole blood theophylline enzyme immunochromatography assay

AU - Habib, Michael P

AU - Schifman, R. B.

AU - Shon, B. Y.

AU - Fiastro, J. F.

AU - Campbell, S. C.

N1 - Funding Information: Supported in part by the Veterans Administration and funds from Syntex Diagnostics, Palo Alto, Calif.

PY - 1987

Y1 - 1987

N2 - A new whole blood enzyme immunochromatographic (EIC) theophylline assay was evaluated in 18 low (25 to 37 percent) and 15 high hematocrit (49 to 56 percent) samples. A good correlation was observed between EIC and fluorescence polarization methods for plasma samples (r = 0.95). However, comparison of results between EIC whole blood and plasma values demonstrates a significant proportional bias that is inversely related to the sample's hematocrit. The EIC method for whole blood samples may substantially underestimate theophylline levels in polycythemic patients with theophylline values near or above the toxic range and underestimate levels in those with anemia, if a correction is not made for the sample's hematocrit. A correction formula to approximate plasma theophylline concentrations from whole blood measurements is described.

AB - A new whole blood enzyme immunochromatographic (EIC) theophylline assay was evaluated in 18 low (25 to 37 percent) and 15 high hematocrit (49 to 56 percent) samples. A good correlation was observed between EIC and fluorescence polarization methods for plasma samples (r = 0.95). However, comparison of results between EIC whole blood and plasma values demonstrates a significant proportional bias that is inversely related to the sample's hematocrit. The EIC method for whole blood samples may substantially underestimate theophylline levels in polycythemic patients with theophylline values near or above the toxic range and underestimate levels in those with anemia, if a correction is not made for the sample's hematocrit. A correction formula to approximate plasma theophylline concentrations from whole blood measurements is described.

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Evaluation of whole blood theophylline enzyme immunochromatography assay

Abstract

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