@inproceedings{c7b3674221074b0bb5988fe3dec2ce76,
title = "Evaluation of tile artifact correction methods for multiphoton microscopy mosaics of whole-slide tissue sections",
abstract = "Multi-photon microscopy (MPM) is a useful biomedical imaging tool due, in part, to its capabilities of probing tissue biomarkers at high resolution and with depth-resolved capabilities. Automated MPM tile scanning allows for whole-slide image acquisition but suffers from tile-stitching artifacts that prevent accurate quantitative data analysis. We have investigated a variety of post-processing artifact correction methods using ImageJ macros and custom Python/ MATLAB code and present a quantitative and qualitative comparison of these methods using whole-slide MPM autofluorescence images of human duodenal tissue. Image quality is assessed via evaluation of artifact removal compared to the calculated mean square error (MSE), peak signal-to-noise ratio (PSNR), and structural similarity index (SSIM) of the processed image and its raw counterpart. Consideration of both quantitative and qualitative results suggest a combination of flat-field based correction and frequency filtering processing steps provide improved artifact correction when compared to each method used independently to correct for tiling artifacts of tile-scan MPM images.",
keywords = "Multiphoton, artifacts, image processing, microscopy, multiwavelength, tiling, whole slide",
author = "Thomas Knapp and Natzem Lima and Suzann Duan and Merchant, {Juanita L.} and Sawyer, {Travis W.}",
note = "Publisher Copyright: {\textcopyright} 2022 SPIE.; Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX 2022 ; Conference date: 20-02-2022 Through 24-02-2022",
year = "2022",
doi = "10.1117/12.2609634",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Brown, {Thomas G.} and Tony Wilson and Laura Waller",
booktitle = "Three-Dimensional and Multidimensional Microscopy",
}