@inproceedings{c7b3674221074b0bb5988fe3dec2ce76,
title = "Evaluation of tile artifact correction methods for multiphoton microscopy mosaics of whole-slide tissue sections",
abstract = "Multi-photon microscopy (MPM) is a useful biomedical imaging tool due, in part, to its capabilities of probing tissue biomarkers at high resolution and with depth-resolved capabilities. Automated MPM tile scanning allows for whole-slide image acquisition but suffers from tile-stitching artifacts that prevent accurate quantitative data analysis. We have investigated a variety of post-processing artifact correction methods using ImageJ macros and custom Python/ MATLAB code and present a quantitative and qualitative comparison of these methods using whole-slide MPM autofluorescence images of human duodenal tissue. Image quality is assessed via evaluation of artifact removal compared to the calculated mean square error (MSE), peak signal-to-noise ratio (PSNR), and structural similarity index (SSIM) of the processed image and its raw counterpart. Consideration of both quantitative and qualitative results suggest a combination of flat-field based correction and frequency filtering processing steps provide improved artifact correction when compared to each method used independently to correct for tiling artifacts of tile-scan MPM images.",
keywords = "Multiphoton, artifacts, image processing, microscopy, multiwavelength, tiling, whole slide",
author = "Thomas Knapp and Natzem Lima and Suzann Duan and Merchant, {Juanita L.} and Sawyer, {Travis W.}",
note = "Funding Information: This work was made possible with the support of Patricia Jansma, Imaging Core Marley at the University of Arizona, NIH Grant GM132008 and the University of Arizona Core Facilities Pilot program. Publisher Copyright: {\textcopyright} 2022 SPIE.; Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX 2022 ; Conference date: 20-02-2022 Through 24-02-2022",
year = "2022",
doi = "10.1117/12.2609634",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Brown, {Thomas G.} and Tony Wilson and Laura Waller",
booktitle = "Three-Dimensional and Multidimensional Microscopy",
}