Evaluation of tile artifact correction methods for multiphoton microscopy mosaics of whole-slide tissue sections

Thomas Knapp, Natzem Lima, Suzann Duan, Juanita L. Merchant, Travis W. Sawyer

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Multi-photon microscopy (MPM) is a useful biomedical imaging tool due, in part, to its capabilities of probing tissue biomarkers at high resolution and with depth-resolved capabilities. Automated MPM tile scanning allows for whole-slide image acquisition but suffers from tile-stitching artifacts that prevent accurate quantitative data analysis. We have investigated a variety of post-processing artifact correction methods using ImageJ macros and custom Python/ MATLAB code and present a quantitative and qualitative comparison of these methods using whole-slide MPM autofluorescence images of human duodenal tissue. Image quality is assessed via evaluation of artifact removal compared to the calculated mean square error (MSE), peak signal-to-noise ratio (PSNR), and structural similarity index (SSIM) of the processed image and its raw counterpart. Consideration of both quantitative and qualitative results suggest a combination of flat-field based correction and frequency filtering processing steps provide improved artifact correction when compared to each method used independently to correct for tiling artifacts of tile-scan MPM images.

Original languageEnglish (US)
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XXIX
EditorsThomas G. Brown, Tony Wilson, Laura Waller
PublisherSPIE
ISBN (Electronic)9781510648036
DOIs
StatePublished - 2022
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX 2022 - Virtual, Online
Duration: Feb 20 2022Feb 24 2022

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11966
ISSN (Print)1605-7422

Conference

ConferenceThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX 2022
CityVirtual, Online
Period2/20/222/24/22

Keywords

  • Multiphoton
  • artifacts
  • image processing
  • microscopy
  • multiwavelength
  • tiling
  • whole slide

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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