Abstract
Preliminary double diffusion gel precipitation studies of guinea pig antisera to antigens of rabbit male accessory glands tissues of reproduction have been successful in demonstrating in vitro freeze-thaw induced alterations of these antigens. Freeze-thaw treatment of these antigens in the presence of the alkylating reagent iodoacetamide resulted in the formation of a precipitate which proved to be refractory to solubilization in 6 m urea or 0.2 m mercaptoethanol, or in a combination of both. The possible mechanisms involved in these alterations are discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 401-403 |
| Number of pages | 3 |
| Journal | Cryobiology |
| Volume | 6 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1970 |
| Externally published | Yes |
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology
- General Agricultural and Biological Sciences
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