Preliminary double diffusion gel precipitation studies of guinea pig antisera to antigens of rabbit male accessory glands tissues of reproduction have been successful in demonstrating in vitro freeze-thaw induced alterations of these antigens. Freeze-thaw treatment of these antigens in the presence of the alkylating reagent iodoacetamide resulted in the formation of a precipitate which proved to be refractory to solubilization in 6 m urea or 0.2 m mercaptoethanol, or in a combination of both. The possible mechanisms involved in these alterations are discussed.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)