Evaluation of the freeze-thaw alteration of rabbit male urogenital tissue antigens by double diffusion gel precipitation

Paul Bronson, R. J. Ablin, Ward A. Soanes

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Preliminary double diffusion gel precipitation studies of guinea pig antisera to antigens of rabbit male accessory glands tissues of reproduction have been successful in demonstrating in vitro freeze-thaw induced alterations of these antigens. Freeze-thaw treatment of these antigens in the presence of the alkylating reagent iodoacetamide resulted in the formation of a precipitate which proved to be refractory to solubilization in 6 m urea or 0.2 m mercaptoethanol, or in a combination of both. The possible mechanisms involved in these alterations are discussed.

Original languageEnglish (US)
Pages (from-to)401-403
Number of pages3
JournalCryobiology
Volume6
Issue number5
DOIs
StatePublished - 1970
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Fingerprint

Dive into the research topics of 'Evaluation of the freeze-thaw alteration of rabbit male urogenital tissue antigens by double diffusion gel precipitation'. Together they form a unique fingerprint.

Cite this