Evaluation of the freeze-thaw alteration of rabbit male urogenital tissue antigens by double diffusion gel precipitation

Paul Bronson; R. J. Ablin; Ward A. Soanes

doi:10.1016/S0011-2240(70)80096-7

Evaluation of the freeze-thaw alteration of rabbit male urogenital tissue antigens by double diffusion gel precipitation

Paul Bronson, R. J. Ablin, Ward A. Soanes

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Preliminary double diffusion gel precipitation studies of guinea pig antisera to antigens of rabbit male accessory glands tissues of reproduction have been successful in demonstrating in vitro freeze-thaw induced alterations of these antigens. Freeze-thaw treatment of these antigens in the presence of the alkylating reagent iodoacetamide resulted in the formation of a precipitate which proved to be refractory to solubilization in 6 m urea or 0.2 m mercaptoethanol, or in a combination of both. The possible mechanisms involved in these alterations are discussed.

Original language	English (US)
Pages (from-to)	401-403
Number of pages	3
Journal	Cryobiology
Volume	6
Issue number	5
DOIs	https://doi.org/10.1016/S0011-2240(70)80096-7
State	Published - 1970
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences

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title = "Evaluation of the freeze-thaw alteration of rabbit male urogenital tissue antigens by double diffusion gel precipitation",

abstract = "Preliminary double diffusion gel precipitation studies of guinea pig antisera to antigens of rabbit male accessory glands tissues of reproduction have been successful in demonstrating in vitro freeze-thaw induced alterations of these antigens. Freeze-thaw treatment of these antigens in the presence of the alkylating reagent iodoacetamide resulted in the formation of a precipitate which proved to be refractory to solubilization in 6 m urea or 0.2 m mercaptoethanol, or in a combination of both. The possible mechanisms involved in these alterations are discussed.",

author = "Paul Bronson and Ablin, {R. J.} and Soanes, {Ward A.}",

note = "Funding Information: Received September 29, 1969. * Supported by a grant from the John A. Itart-ford Foundation. Inc.",

year = "1970",

doi = "10.1016/S0011-2240(70)80096-7",

language = "English (US)",

volume = "6",

pages = "401--403",

journal = "Cryobiology",

issn = "0011-2240",

publisher = "Academic Press Inc.",

number = "5",

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T1 - Evaluation of the freeze-thaw alteration of rabbit male urogenital tissue antigens by double diffusion gel precipitation

AU - Bronson, Paul

AU - Ablin, R. J.

AU - Soanes, Ward A.

N1 - Funding Information: Received September 29, 1969. * Supported by a grant from the John A. Itart-ford Foundation. Inc.

PY - 1970

Y1 - 1970

N2 - Preliminary double diffusion gel precipitation studies of guinea pig antisera to antigens of rabbit male accessory glands tissues of reproduction have been successful in demonstrating in vitro freeze-thaw induced alterations of these antigens. Freeze-thaw treatment of these antigens in the presence of the alkylating reagent iodoacetamide resulted in the formation of a precipitate which proved to be refractory to solubilization in 6 m urea or 0.2 m mercaptoethanol, or in a combination of both. The possible mechanisms involved in these alterations are discussed.

AB - Preliminary double diffusion gel precipitation studies of guinea pig antisera to antigens of rabbit male accessory glands tissues of reproduction have been successful in demonstrating in vitro freeze-thaw induced alterations of these antigens. Freeze-thaw treatment of these antigens in the presence of the alkylating reagent iodoacetamide resulted in the formation of a precipitate which proved to be refractory to solubilization in 6 m urea or 0.2 m mercaptoethanol, or in a combination of both. The possible mechanisms involved in these alterations are discussed.

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U2 - 10.1016/S0011-2240(70)80096-7

DO - 10.1016/S0011-2240(70)80096-7

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AN - SCOPUS:0014755053

SN - 0011-2240

VL - 6

SP - 401

EP - 403

JO - Cryobiology

JF - Cryobiology

IS - 5

ER -

Evaluation of the freeze-thaw alteration of rabbit male urogenital tissue antigens by double diffusion gel precipitation

Abstract

ASJC Scopus subject areas

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