Evaluation of illumination systems for wide-field hyperspectral imaging in biomedical applications

Travis W. Sawyer, A. Siri Luthman, Sarah E. Bohndiek

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Hyperspectral imaging (HSI) systems collect both morphological and chemical characteristics from a sample by simultaneously acquiring spatial and spectral information. HSI has potential to advance cancer diagnostics by characterizing reectance and uorescence properties of a tissue, as well as extracting microstructural in- formation, all of which are altered through the development of a tumor. Illumination uniformity is a critical pre-condition for extracting quantitative data from an HSI system. Spatial, angular, or spectral non-uniformity can cause glare, specular reection and unwanted shading, which negatively impact statistical analysis tech- niques used to extract abundance of different chemical species. This is further exacerbated when imaging three-dimensional structures, such as tumors, whose appearance can cast shadows and form other occlusions. Furthermore, as HSI can be used simultaneously for white light and uorescence imaging, a exible system, which multiplexes narrowband and broadband illumination is necessary to fully utilize the capabilities of a biomedical HSI system. To address these challenges, we modeled illumination systems frequently used in wide-field biological imaging with the software LightTools and FRED. Each system is characterized for spectral, spatial, and angular uniformity, as well as total effciency. While all three systems provide high spatial and spectral uniformity, the highest angular uniformity is achieved using a diffiuse scattering dome, yielding a contrast of 0.503 and average deviation of 0.303 with a 3.91% model error. Nonetheless, results suggest that conventional systems may not be suitable for low-light-level applications, where tailoring illumination to match spatial and spectral requirements may be the best approach to maximize the performance.

Original languageEnglish (US)
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV
EditorsDaniel L. Farkas, Dan V. Nicolau, Robert C. Leif
PublisherSPIE
ISBN (Electronic)9781510605770
DOIs
StatePublished - 2017
Externally publishedYes
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV - San Francisco, United States
Duration: Jan 30 2017Feb 1 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10068
ISSN (Print)1605-7422

Conference

ConferenceImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV
Country/TerritoryUnited States
CitySan Francisco
Period1/30/172/1/17

Keywords

  • Hyperspectral imaging
  • Illumination
  • Modeling
  • Simulation
  • Uniformity

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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