@inbook{3db25053641d41e2a550b789e9400798,
title = "Evaluating the Activity of Ras/Rap GTPases in Dictyostelium",
abstract = "Ras and Rap small GTPases of the Ras superfamily act as molecular switches to control diverse cellular processes as part of different signaling pathways. Dictyostelium expresses several Ras and Rap proteins, and their study has and continues to greatly contribute to our understanding of their role in eukaryote biology. To study the activity of Ras and Rap proteins in Dictyostelium, several assays based on their interaction with the Ras binding domain of known eukaryotic Ras/Rap effectors have been developed and proved extremely useful to study their regulation and cellular roles. Here, we describe methods to assess Ras/Rap activity biochemically using a pull-down assay and through live-cell imaging using fluorescent reporters.",
keywords = "Activity assays, Dictyostelium discoideum, Imaging, Pull-down, Rap1, Ras, Ras binding domain",
author = "Smith, {Stephen F.} and Charest, {Pascale G.}",
note = "Publisher Copyright: {\textcopyright} The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "10.1007/978-1-0716-3894-1_12",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "163--176",
booktitle = "Methods in Molecular Biology",
}