Euglena gracilis chloroplast psbB, psbT, psbH and psbN gene cluster: Regulation of psbB-psbT pre-mRNA processing

Ling Hong; Jennifer K. Stevenson; W. Barry Roth; Richard B. Hallick

doi:10.1007/BF00705648

Euglena gracilis chloroplast psbB, psbT, psbH and psbN gene cluster: Regulation of psbB-psbT pre-mRNA processing

Ling Hong, Jennifer K. Stevenson, W. Barry Roth, Richard B. Hallick

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

A 2.4 kb region of the Euglena gracilis chloroplast genome containing the genes psbT, psbH and psbN was characterized. The mRNAs transcribed from psbB, psbT, psbH and psbN were analyzed by northern hybridization, S1 nuclease protection analysis and primer extension RNA sequencing. The gene pairs psbB psbT and psbH-psbN are cotranscribed from opposite strands. The 5′ end of the psbN-psbH transcript and the intercistronic cleavage sites between psbB-psbT and psbN-psbH were determined. The extent of psbB-psbT intercistronic cleavage is greater during photoautotrophic than heterotrophic growth and thus may be developmentally regulated. Processing is absent in the nonphotosynthetic E. gracilis mutant Y9Z1NaL.

Original language	English (US)
Pages (from-to)	180-188
Number of pages	9
Journal	MGG Molecular & General Genetics
Volume	247
Issue number	2
DOIs	https://doi.org/10.1007/BF00705648
State	Published - Mar 1995
Externally published	Yes

Keywords

Euglena gracilis chloroplast
Evolution of psbB operon
Light induction
Photosystem II polypeptides
RNA processing

ASJC Scopus subject areas

Genetics

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10.1007/BF00705648

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title = "Euglena gracilis chloroplast psbB, psbT, psbH and psbN gene cluster: Regulation of psbB-psbT pre-mRNA processing",

abstract = "A 2.4 kb region of the Euglena gracilis chloroplast genome containing the genes psbT, psbH and psbN was characterized. The mRNAs transcribed from psbB, psbT, psbH and psbN were analyzed by northern hybridization, S1 nuclease protection analysis and primer extension RNA sequencing. The gene pairs psbB psbT and psbH-psbN are cotranscribed from opposite strands. The 5′ end of the psbN-psbH transcript and the intercistronic cleavage sites between psbB-psbT and psbN-psbH were determined. The extent of psbB-psbT intercistronic cleavage is greater during photoautotrophic than heterotrophic growth and thus may be developmentally regulated. Processing is absent in the nonphotosynthetic E. gracilis mutant Y9Z1NaL.",

keywords = "Euglena gracilis chloroplast, Evolution of psbB operon, Light induction, Photosystem II polypeptides, RNA processing",

author = "Ling Hong and Stevenson, {Jennifer K.} and Roth, {W. Barry} and Hallick, {Richard B.}",

year = "1995",

month = mar,

doi = "10.1007/BF00705648",

language = "English (US)",

volume = "247",

pages = "180--188",

journal = "MGG Molecular & General Genetics",

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T1 - Euglena gracilis chloroplast psbB, psbT, psbH and psbN gene cluster

T2 - Regulation of psbB-psbT pre-mRNA processing

AU - Hong, Ling

AU - Stevenson, Jennifer K.

AU - Roth, W. Barry

AU - Hallick, Richard B.

PY - 1995/3

Y1 - 1995/3

N2 - A 2.4 kb region of the Euglena gracilis chloroplast genome containing the genes psbT, psbH and psbN was characterized. The mRNAs transcribed from psbB, psbT, psbH and psbN were analyzed by northern hybridization, S1 nuclease protection analysis and primer extension RNA sequencing. The gene pairs psbB psbT and psbH-psbN are cotranscribed from opposite strands. The 5′ end of the psbN-psbH transcript and the intercistronic cleavage sites between psbB-psbT and psbN-psbH were determined. The extent of psbB-psbT intercistronic cleavage is greater during photoautotrophic than heterotrophic growth and thus may be developmentally regulated. Processing is absent in the nonphotosynthetic E. gracilis mutant Y9Z1NaL.

AB - A 2.4 kb region of the Euglena gracilis chloroplast genome containing the genes psbT, psbH and psbN was characterized. The mRNAs transcribed from psbB, psbT, psbH and psbN were analyzed by northern hybridization, S1 nuclease protection analysis and primer extension RNA sequencing. The gene pairs psbB psbT and psbH-psbN are cotranscribed from opposite strands. The 5′ end of the psbN-psbH transcript and the intercistronic cleavage sites between psbB-psbT and psbN-psbH were determined. The extent of psbB-psbT intercistronic cleavage is greater during photoautotrophic than heterotrophic growth and thus may be developmentally regulated. Processing is absent in the nonphotosynthetic E. gracilis mutant Y9Z1NaL.

KW - Euglena gracilis chloroplast

KW - Evolution of psbB operon

KW - Light induction

KW - Photosystem II polypeptides

KW - RNA processing

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U2 - 10.1007/BF00705648

DO - 10.1007/BF00705648

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AN - SCOPUS:0028923482

SN - 0026-8925

VL - 247

SP - 180

EP - 188

JO - MGG Molecular & General Genetics

JF - MGG Molecular & General Genetics

IS - 2

ER -

Euglena gracilis chloroplast psbB, psbT, psbH and psbN gene cluster: Regulation of psbB-psbT pre-mRNA processing

Abstract

Keywords

ASJC Scopus subject areas

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