Abstract
Ethanol administration has been shown to affect β-endorphin (β-E) levels in most brain areas. Chronic ethanol treatment has also lead to changes in the levels of Met- and Leu-enkephalin which may be due to the recent finding that enkephalinase A activity is significantly altered. To determine if proteolytic enzymes responsible for β-E metabolism at the pSPM are also altered, we studied the effect of chronic ethanol (7% v/v; 8 days) administration on in vitro central β-E metabolism in male C57/BL mice. Purified SPM was time-course incubated with β-E (20 μM) for 30-120 min and subjected to HPLC analyses for determination of β-endorphin and related fragments. Chronic ethanol significantly reduced the half-life for β-E at the pSPM (T 1 2=50/min) versus controls (T 1 2=100.4 min). Chronic ethanol also caused significant accumulation of the behaviorally active α- and γ-type endorphins formed at the pSPM. These results suggest that chronic ethanol treatment leads to an increase in the activity of peptidases responsible for β-E metabolism at pSPM leading to an increased formation of both α- and γ-type endorphins which may affect alcohol related behaviors.
Original language | English (US) |
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Pages (from-to) | 467-472 |
Number of pages | 6 |
Journal | Peptides |
Volume | 8 |
Issue number | 3 |
DOIs | |
State | Published - 1987 |
Keywords
- Ethanol
- HPLC
- Metabolism
- α- and γ-type endorphins
- β-Endorphin
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Endocrinology
- Cellular and Molecular Neuroscience