Estimation of the dissociation constant of naloxone in the naive and morphine-tolerant guinea-pig isolated ileum: Analysis by the constrained schild plot

Ronald J. Tallarida; Mary Jane Robinson; Frank Porreca; Alan Cowan

doi:10.1016/0024-3205(82)90187-4

Estimation of the dissociation constant of naloxone in the naive and morphine-tolerant guinea-pig isolated ileum: Analysis by the constrained schild plot

Ronald J. Tallarida, Mary Jane Robinson, Frank Porreca, Alan Cowan

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The pA₂ values for naloxone-morphine were determined using ilea taken from naive and morphine-tolerant guinea-pigs. This constant decreased from 8.56 (±0.22) in the naive to 7.95 (±0.12) in tolerant tissues. The values of the pA₂'s were based on Schild plots using conventional regression techniques; this regression resulted in values of -log K_B of 8.79 (±1.5) and 8.18 (±1.2) in naive and morphine-tolerant ilea, respectively. While pA₂ differed, the difference in -log K_B was not statistically significant. Since these two values are theoretically equivalent, the above finding appears paradoxical. Constraining the slope of the Schild plot to the theoretically required -1 resolved this discrepancy by reducing the error inherent in the calculation of -log K_B determined by conventional regression.

Original language	English (US)
Pages (from-to)	1691-1694
Number of pages	4
Journal	Life Sciences
Volume	31
Issue number	16-17
DOIs	https://doi.org/10.1016/0024-3205(82)90187-4
State	Published - 1982
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
Pharmacology, Toxicology and Pharmaceutics(all)

Access to Document

10.1016/0024-3205(82)90187-4

Cite this

@article{9a5ce2f95c544eee93c290249c324ef7,

title = "Estimation of the dissociation constant of naloxone in the naive and morphine-tolerant guinea-pig isolated ileum: Analysis by the constrained schild plot",

abstract = "The pA2 values for naloxone-morphine were determined using ilea taken from naive and morphine-tolerant guinea-pigs. This constant decreased from 8.56 (±0.22) in the naive to 7.95 (±0.12) in tolerant tissues. The values of the pA2's were based on Schild plots using conventional regression techniques; this regression resulted in values of -log KB of 8.79 (±1.5) and 8.18 (±1.2) in naive and morphine-tolerant ilea, respectively. While pA2 differed, the difference in -log KB was not statistically significant. Since these two values are theoretically equivalent, the above finding appears paradoxical. Constraining the slope of the Schild plot to the theoretically required -1 resolved this discrepancy by reducing the error inherent in the calculation of -log KB determined by conventional regression.",

author = "Tallarida, {Ronald J.} and {Jane Robinson}, Mary and Frank Porreca and Alan Cowan",

year = "1982",

doi = "10.1016/0024-3205(82)90187-4",

language = "English (US)",

volume = "31",

pages = "1691--1694",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "16-17",

}

TY - JOUR

T1 - Estimation of the dissociation constant of naloxone in the naive and morphine-tolerant guinea-pig isolated ileum

T2 - Analysis by the constrained schild plot

AU - Tallarida, Ronald J.

AU - Jane Robinson, Mary

AU - Porreca, Frank

AU - Cowan, Alan

PY - 1982

Y1 - 1982

N2 - The pA2 values for naloxone-morphine were determined using ilea taken from naive and morphine-tolerant guinea-pigs. This constant decreased from 8.56 (±0.22) in the naive to 7.95 (±0.12) in tolerant tissues. The values of the pA2's were based on Schild plots using conventional regression techniques; this regression resulted in values of -log KB of 8.79 (±1.5) and 8.18 (±1.2) in naive and morphine-tolerant ilea, respectively. While pA2 differed, the difference in -log KB was not statistically significant. Since these two values are theoretically equivalent, the above finding appears paradoxical. Constraining the slope of the Schild plot to the theoretically required -1 resolved this discrepancy by reducing the error inherent in the calculation of -log KB determined by conventional regression.

AB - The pA2 values for naloxone-morphine were determined using ilea taken from naive and morphine-tolerant guinea-pigs. This constant decreased from 8.56 (±0.22) in the naive to 7.95 (±0.12) in tolerant tissues. The values of the pA2's were based on Schild plots using conventional regression techniques; this regression resulted in values of -log KB of 8.79 (±1.5) and 8.18 (±1.2) in naive and morphine-tolerant ilea, respectively. While pA2 differed, the difference in -log KB was not statistically significant. Since these two values are theoretically equivalent, the above finding appears paradoxical. Constraining the slope of the Schild plot to the theoretically required -1 resolved this discrepancy by reducing the error inherent in the calculation of -log KB determined by conventional regression.

UR - http://www.scopus.com/inward/record.url?scp=0020432818&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020432818&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(82)90187-4

DO - 10.1016/0024-3205(82)90187-4

M3 - Article

C2 - 7154829

AN - SCOPUS:0020432818

SN - 0024-3205

VL - 31

SP - 1691

EP - 1694

JO - Life Sciences

JF - Life Sciences

IS - 16-17

ER -

Estimation of the dissociation constant of naloxone in the naive and morphine-tolerant guinea-pig isolated ileum: Analysis by the constrained schild plot

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this