Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from Organoids

Emma Teal, Nina Bertaux-Skeirik, Jayati Chakrabarti, Loryn Holokai, Yana Zavros

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Organoid cultures generated from gastrointestinal tissues have been an invaluable advancement for in vitro studies of physiological function and disease. Here we present a comprehensive protocol for the establishment and culture of human- and mouse-derived 3-dimensional gastric organoids transferred to 2-dimensional gastric epithelial cell monolayers. We introduce two methods that include the establishment of monolayers from: (1) intact organoids, and (2) single cells dissociated from intact organoids.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages145-155
Number of pages11
DOIs
StatePublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1817
ISSN (Print)1064-3745

Keywords

  • Gastric organoids
  • Monolayer
  • Regeneration
  • Repair
  • Scratch wound assay
  • Stem cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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