@inbook{686004a34de44f0f9236035805760cc6,
title = "Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from Organoids",
abstract = "Organoid cultures generated from gastrointestinal tissues have been an invaluable advancement for in vitro studies of physiological function and disease. Here we present a comprehensive protocol for the establishment and culture of human- and mouse-derived 3-dimensional gastric organoids transferred to 2-dimensional gastric epithelial cell monolayers. We introduce two methods that include the establishment of monolayers from: (1) intact organoids, and (2) single cells dissociated from intact organoids.",
keywords = "Gastric organoids, Monolayer, Regeneration, Repair, Scratch wound assay, Stem cells",
author = "Emma Teal and Nina Bertaux-Skeirik and Jayati Chakrabarti and Loryn Holokai and Yana Zavros",
note = "Funding Information: This work is financially supported by NIH5R01DK083402-08 grant (Zavros). Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2018",
doi = "10.1007/978-1-4939-8600-2_15",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "145--155",
booktitle = "Methods in Molecular Biology",
}