TY - JOUR
T1 - Essential role of bone marrow fibroblast growth factor-2 in the effect of estradiol on reendothelialization and endothelial progenitor cell mobilization
AU - Fontaine, Vincent
AU - Filipe, Cédric
AU - Werner, Nikos
AU - Gourdy, Pierre
AU - Billon, Audrey
AU - Garmy-Susini, Barbara
AU - Brouchet, Laurent
AU - Bayard, Francis
AU - Prats, Hervé
AU - Doetschman, Thomas
AU - Nickenig, Georg
AU - Arnal, Jean François
N1 - Funding Information:
The work was supported in part by INSERM, Université Paul Sabatier Toulouse III, the European Vascular Genomics Network No. 503254, the Agence Nationale de la Recherche, the Fondation de France, the Fondation de l'Avenir, and the Conseil Régional Midi-Pyrénées in France. V.F. was supported by a grant from the Nouvelle Société Française d'Athérosclérose.
PY - 2006/11
Y1 - 2006/11
N2 - 17β-Estradiol (E2) accelerates reendothelialization and increases the number of circulating endothelial progenitor cells (EPCs), but whether fibroblast growth factor-2 (FGF2) is involved in these processes remains unknown. Here we explored the role of FGF2 in the effect of E2 on reendothelialization and EPC levels in a mouse model. As previously reported, E2 increased both the velocity of reendothelialization and the number of circulating EPCs in ovariectomized wild-type (Fgf2+/+) mice. In contrast, the effect of E2 on both parameters was abolished in FGF2-deficient mice (Fgf2-/-), demonstrating that FGF2 is absolutely required for these effects of E2. To test the implication of medullary and extramedullary FGF2, we developed chimeric mice by grafting Fgf2-/- bone marrow to Fgf2+/+ [Fgf2-/- bone marrow (BM) = >Fgf2+/+] mice and observed that the effect of E2 on both reendothelialization and EPC levels was abolished. In contrast, both effects of E2 in Fgf2+/+BM = >Fgf2 -/- mice were similar to those observed in Fgf2+/+ mice, demonstrating that only BM-derived, but not extramedullary, FGF2 is required for both effects. Interestingly, E2 was found to markedly increase both FGF2lmw and FGF2hmw in bone marrow. In conclusion, FGF2, specifically medullary FGF2, is necessary and sufficient to mediate the accelerative effect of E2 on both reendothelialization and EPC mobilization.
AB - 17β-Estradiol (E2) accelerates reendothelialization and increases the number of circulating endothelial progenitor cells (EPCs), but whether fibroblast growth factor-2 (FGF2) is involved in these processes remains unknown. Here we explored the role of FGF2 in the effect of E2 on reendothelialization and EPC levels in a mouse model. As previously reported, E2 increased both the velocity of reendothelialization and the number of circulating EPCs in ovariectomized wild-type (Fgf2+/+) mice. In contrast, the effect of E2 on both parameters was abolished in FGF2-deficient mice (Fgf2-/-), demonstrating that FGF2 is absolutely required for these effects of E2. To test the implication of medullary and extramedullary FGF2, we developed chimeric mice by grafting Fgf2-/- bone marrow to Fgf2+/+ [Fgf2-/- bone marrow (BM) = >Fgf2+/+] mice and observed that the effect of E2 on both reendothelialization and EPC levels was abolished. In contrast, both effects of E2 in Fgf2+/+BM = >Fgf2 -/- mice were similar to those observed in Fgf2+/+ mice, demonstrating that only BM-derived, but not extramedullary, FGF2 is required for both effects. Interestingly, E2 was found to markedly increase both FGF2lmw and FGF2hmw in bone marrow. In conclusion, FGF2, specifically medullary FGF2, is necessary and sufficient to mediate the accelerative effect of E2 on both reendothelialization and EPC mobilization.
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U2 - 10.2353/ajpath.2006.060260
DO - 10.2353/ajpath.2006.060260
M3 - Article
C2 - 17071606
AN - SCOPUS:34250327362
SN - 0002-9440
VL - 169
SP - 1855
EP - 1862
JO - American Journal of Pathology
JF - American Journal of Pathology
IS - 5
ER -