Errors in confocal fluorescence ratiometric imaging microscopy due to chromatic aberration

Yuxiang Lin; Arthur F. Gmitro

doi:10.1364/AO.50.000095

Errors in confocal fluorescence ratiometric imaging microscopy due to chromatic aberration

Yuxiang Lin, Arthur F. Gmitro

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Confocal fluorescence ratiometric imaging is an optical technique used to measure a variety of important biological parameters. A small amount of chromatic aberration in the microscope system can introduce a variation in the signal ratio dependent on the fluorophore concentration gradient along the optical axis and lead to bias in the measurement. We present a theoretical model of this effect. Experimental results and simulations clearly demonstrate that this error can be significant and should not be ignored.

Original language	English (US)
Pages (from-to)	95-102
Number of pages	8
Journal	Applied optics
Volume	50
Issue number	1
DOIs	https://doi.org/10.1364/AO.50.000095
State	Published - Jan 1 2011

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Engineering (miscellaneous)
Electrical and Electronic Engineering

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10.1364/AO.50.000095

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abstract = "Confocal fluorescence ratiometric imaging is an optical technique used to measure a variety of important biological parameters. A small amount of chromatic aberration in the microscope system can introduce a variation in the signal ratio dependent on the fluorophore concentration gradient along the optical axis and lead to bias in the measurement. We present a theoretical model of this effect. Experimental results and simulations clearly demonstrate that this error can be significant and should not be ignored.",

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AB - Confocal fluorescence ratiometric imaging is an optical technique used to measure a variety of important biological parameters. A small amount of chromatic aberration in the microscope system can introduce a variation in the signal ratio dependent on the fluorophore concentration gradient along the optical axis and lead to bias in the measurement. We present a theoretical model of this effect. Experimental results and simulations clearly demonstrate that this error can be significant and should not be ignored.

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Errors in confocal fluorescence ratiometric imaging microscopy due to chromatic aberration

Abstract

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