Epitope mapping of the human papillomavirus type 16 E4 protein by means of synthetic peptides

A. Suchankova; V. Krchnak; J. Vagner; E. Hamsikova; M. Krcmar; L. Ritterova; V. Vonka

doi:10.1099/0022-1317-73-2-429

Epitope mapping of the human papillomavirus type 16 E4 protein by means of synthetic peptides

A. Suchankova, V. Krchnak, J. Vagner, E. Hamsikova, M. Krcmar, L. Ritterova, V. Vonka

Research output: Contribution to journal › Article › peer-review

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Abstract

Eight overlapping icosapeptides covering the entire sequence of the E4 protein of human papillomavirus type 16 (HPV-16), were prepared and tested for their reactivity with human sera in IgG-specific ELISA. The strongest reactivity of sera from HPV-16 DNA-positive invasive cervical carcinoma (INCA) patients was detected with the peptide denoted 16/E4-6, covering amino acids 51 to 70. Subsequently nearly 200 sera were tested for the presence of the 16/E4-6-specific antibody. Reactivity was more frequent in cervical intraepithelial neoplasia patients and INCA patients than in matched control subjects. Sera from INCA patients were also tested for antibody reactive with peptide 16/E7-2 covering the major type-specific reactive region of the HPV- 16/E7 protein. Only four of 13 sera possessing the 16/E4-6-specific antibody were reactive with the 16/E7-2 peptide.

Original language	English (US)
Pages (from-to)	429-432
Number of pages	4
Journal	Journal of General Virology
Volume	73
Issue number	2
DOIs	https://doi.org/10.1099/0022-1317-73-2-429
State	Published - 1992
Externally published	Yes

ASJC Scopus subject areas

Virology

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title = "Epitope mapping of the human papillomavirus type 16 E4 protein by means of synthetic peptides",

abstract = "Eight overlapping icosapeptides covering the entire sequence of the E4 protein of human papillomavirus type 16 (HPV-16), were prepared and tested for their reactivity with human sera in IgG-specific ELISA. The strongest reactivity of sera from HPV-16 DNA-positive invasive cervical carcinoma (INCA) patients was detected with the peptide denoted 16/E4-6, covering amino acids 51 to 70. Subsequently nearly 200 sera were tested for the presence of the 16/E4-6-specific antibody. Reactivity was more frequent in cervical intraepithelial neoplasia patients and INCA patients than in matched control subjects. Sera from INCA patients were also tested for antibody reactive with peptide 16/E7-2 covering the major type-specific reactive region of the HPV- 16/E7 protein. Only four of 13 sera possessing the 16/E4-6-specific antibody were reactive with the 16/E7-2 peptide.",

author = "A. Suchankova and V. Krchnak and J. Vagner and E. Hamsikova and M. Krcmar and L. Ritterova and V. Vonka",

year = "1992",

doi = "10.1099/0022-1317-73-2-429",

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T1 - Epitope mapping of the human papillomavirus type 16 E4 protein by means of synthetic peptides

AU - Suchankova, A.

AU - Krchnak, V.

AU - Vagner, J.

AU - Hamsikova, E.

AU - Krcmar, M.

AU - Ritterova, L.

AU - Vonka, V.

PY - 1992

Y1 - 1992

N2 - Eight overlapping icosapeptides covering the entire sequence of the E4 protein of human papillomavirus type 16 (HPV-16), were prepared and tested for their reactivity with human sera in IgG-specific ELISA. The strongest reactivity of sera from HPV-16 DNA-positive invasive cervical carcinoma (INCA) patients was detected with the peptide denoted 16/E4-6, covering amino acids 51 to 70. Subsequently nearly 200 sera were tested for the presence of the 16/E4-6-specific antibody. Reactivity was more frequent in cervical intraepithelial neoplasia patients and INCA patients than in matched control subjects. Sera from INCA patients were also tested for antibody reactive with peptide 16/E7-2 covering the major type-specific reactive region of the HPV- 16/E7 protein. Only four of 13 sera possessing the 16/E4-6-specific antibody were reactive with the 16/E7-2 peptide.

AB - Eight overlapping icosapeptides covering the entire sequence of the E4 protein of human papillomavirus type 16 (HPV-16), were prepared and tested for their reactivity with human sera in IgG-specific ELISA. The strongest reactivity of sera from HPV-16 DNA-positive invasive cervical carcinoma (INCA) patients was detected with the peptide denoted 16/E4-6, covering amino acids 51 to 70. Subsequently nearly 200 sera were tested for the presence of the 16/E4-6-specific antibody. Reactivity was more frequent in cervical intraepithelial neoplasia patients and INCA patients than in matched control subjects. Sera from INCA patients were also tested for antibody reactive with peptide 16/E7-2 covering the major type-specific reactive region of the HPV- 16/E7 protein. Only four of 13 sera possessing the 16/E4-6-specific antibody were reactive with the 16/E7-2 peptide.

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Epitope mapping of the human papillomavirus type 16 E4 protein by means of synthetic peptides

Abstract

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