Abstract
During early cardiac development, the progenitor cells of the heart valves and membranous septa undergo an epithelial-mesenchymal transformation. Previous studies have shown that this transformation depends on the activity of a transforming growth factor β (TGF/8) molecule produced by the heart. In the present study, we have used modified antisense oligodeoxynucleotides generated to nonconserved regions of TGFβ1, -2, -3, and -4 to examine the possible roles of these members in this transformation. A phosphoramidate-modified oligonucleotide complementary to TGF/33 mRNA was capable of inhibiting normal epithelial-mesenchymal transformation by 80%. Unmodified oligonucleotides to TGFβ3, modified oligonucleotides to TGFβ1, -2, and -4, and two modified control oligonucleotides were unable to inhibit the transformation. These data demonstrate that a specific member of the TGFβ family, TGFβ3, is essential for the epithelialmesenchymal cell transformation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1516-1520 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 88 |
| Issue number | 4 |
| State | Published - 1991 |
Keywords
- Cardiac development
- Growth factors
- Tissue interaction
ASJC Scopus subject areas
- General