Epidermal growth factor and transforming growth factor-α mRNA in rat small intestine: In situ hybridization study

Bohuslav Dvořák; Hana Holubec; Albert V. LeBouton; Jean M. Wilson; Otakar Koldovský

doi:10.1016/0014-5793(94)00942-2

Epidermal growth factor and transforming growth factor-α mRNA in rat small intestine: In situ hybridization study

Bohuslav Dvořák, Hana Holubec, Albert V. LeBouton, Jean M. Wilson, Otakar Koldovský

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The expression of epidermal growth factor (EGF) and transforming growth factor-α (TGF-α) mRNA in the small intestine of suckling and adult rats was examined by in situ hybridization. EGF mRNA was found mainly in the intestinal crypts in adult rats. Adult rats also exhibited a considerably stronger signal for EGF mRNA in comparison to suckling rats, where the signal was very low or absent. In contrast to EGF, very strong expression of TGF-α mRNA was observed in the small intestine of both adult and suckling rats. These data suggest the differences between the expression of EGF and TGF-α in the developing small intestine.

Original language	English (US)
Pages (from-to)	291-295
Number of pages	5
Journal	FEBS Letters
Volume	352
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(94)00942-2
State	Published - Oct 3 1994

Keywords

Epidermal growth factor
Gastrointestinal tract
In situ hybridization
Transforming growth factor-α
submandibular gland

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(94)00942-2

Cite this

@article{6520a533f99245df86822b7eec45fc23,

title = "Epidermal growth factor and transforming growth factor-α mRNA in rat small intestine: In situ hybridization study",

abstract = "The expression of epidermal growth factor (EGF) and transforming growth factor-α (TGF-α) mRNA in the small intestine of suckling and adult rats was examined by in situ hybridization. EGF mRNA was found mainly in the intestinal crypts in adult rats. Adult rats also exhibited a considerably stronger signal for EGF mRNA in comparison to suckling rats, where the signal was very low or absent. In contrast to EGF, very strong expression of TGF-α mRNA was observed in the small intestine of both adult and suckling rats. These data suggest the differences between the expression of EGF and TGF-α in the developing small intestine.",

keywords = "Epidermal growth factor, Gastrointestinal tract, In situ hybridization, Transforming growth factor-α, submandibular gland",

author = "Bohuslav Dvo{\v r}{\'a}k and Hana Holubec and LeBouton, {Albert V.} and Wilson, {Jean M.} and Otakar Koldovsk{\'y}",

year = "1994",

month = oct,

day = "3",

doi = "10.1016/0014-5793(94)00942-2",

language = "English (US)",

volume = "352",

pages = "291--295",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "3",

}

TY - JOUR

T1 - Epidermal growth factor and transforming growth factor-α mRNA in rat small intestine

T2 - In situ hybridization study

AU - Dvořák, Bohuslav

AU - Holubec, Hana

AU - LeBouton, Albert V.

AU - Wilson, Jean M.

AU - Koldovský, Otakar

PY - 1994/10/3

Y1 - 1994/10/3

N2 - The expression of epidermal growth factor (EGF) and transforming growth factor-α (TGF-α) mRNA in the small intestine of suckling and adult rats was examined by in situ hybridization. EGF mRNA was found mainly in the intestinal crypts in adult rats. Adult rats also exhibited a considerably stronger signal for EGF mRNA in comparison to suckling rats, where the signal was very low or absent. In contrast to EGF, very strong expression of TGF-α mRNA was observed in the small intestine of both adult and suckling rats. These data suggest the differences between the expression of EGF and TGF-α in the developing small intestine.

AB - The expression of epidermal growth factor (EGF) and transforming growth factor-α (TGF-α) mRNA in the small intestine of suckling and adult rats was examined by in situ hybridization. EGF mRNA was found mainly in the intestinal crypts in adult rats. Adult rats also exhibited a considerably stronger signal for EGF mRNA in comparison to suckling rats, where the signal was very low or absent. In contrast to EGF, very strong expression of TGF-α mRNA was observed in the small intestine of both adult and suckling rats. These data suggest the differences between the expression of EGF and TGF-α in the developing small intestine.

KW - Epidermal growth factor

KW - Gastrointestinal tract

KW - In situ hybridization

KW - Transforming growth factor-α

KW - submandibular gland

UR - http://www.scopus.com/inward/record.url?scp=0028093009&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028093009&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(94)00942-2

DO - 10.1016/0014-5793(94)00942-2

M3 - Article

C2 - 7925989

AN - SCOPUS:0028093009

SN - 0014-5793

VL - 352

SP - 291

EP - 295

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Epidermal growth factor and transforming growth factor-α mRNA in rat small intestine: In situ hybridization study

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this