Eotaxin Augments Calcification in Vascular Smooth Muscle Cells

Gayatri Raghuraman, Joseph Hsiung, Mary C. Zuniga, Brittanie D. Baughman, Elizabeth Hitchner, Raul J. Guzman, Wei Zhou

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Calcification of atherosclerotic plaques in elderly patients represents a potent risk marker of cardiovascular events. Plasma analyses of patients with or without calcified plaques reveal significant differences in chemokines, particularly eotaxin, which escalates with increased calcification. We therefore, hypothesize that eotaxin in circulation augments calcification of vascular smooth muscle cells (VSMCs) possibly via oxidative stress in the vasculature. We observe that eotaxin increases the rate of calcification significantly in VSMCs as evidenced by increased alkaline phosphatase activity, calcium deposition, and osteogenic marker expression. In addition, eotaxin promotes proliferation in VSMCs and triggers oxidative stress in a NADPH oxidase dependent manner. These primary novel observations support our proposition that in the vasculature eotaxin augments mineralization. Our findings suggest that eotaxin may represent a potential therapeutic target for prevention of cardiovascular complications in the elderly. J. Cell. Biochem. 118: 647–654, 2017.

Original languageEnglish (US)
Pages (from-to)647-654
Number of pages8
JournalJournal of Cellular Biochemistry
Volume118
Issue number3
DOIs
StatePublished - Mar 1 2017
Externally publishedYes

Keywords

  • EOTAXIN
  • NADPH-OXIDASE
  • OXIDATIVE STRESS
  • VASCULAR CALCIFICATION
  • VASCULAR SMOOTH MUSCLE CELL

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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