Enterovirus detection in water with gene probes

P. Gerba; B. Margolin; E. Trumper

Enterovirus detection in water with gene probes

P. Gerba, B. Margolin, E. Trumper

Research output: Contribution to journal › Article › peer-review

Abstract

Water virology has long aimed at the goal of the development of rapid and low cost methods for the detection of enteric viruses in water. Gene probes are small pieces of nucleic acid (either DNA or RNA) that hybridize to the complementary base pairs of specific genes and can be used to identify the genetic material of any organism. We report the development of cDNA probes against poliovirus and hepatitis A virus labeled with ³²PdATP and ³²PdCTP which is capable of detecting as little as one fg of nucleic acid or one tissue culture infective dose of poliovirus or one radio-immuno-focus unit of hepatitis A virus within 72 hours.

Original language	English (US)
Pages (from-to)	518-519
Number of pages	2
Journal	Zeitschrift fur die Gesamte Hygiene und Ihre Grenzgebiete
Volume	34
Issue number	9
State	Published - 1988

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health

Cite this

@article{b4e1c9f288824d69a0ce1f9dcd9a6ff5,

title = "Enterovirus detection in water with gene probes",

abstract = "Water virology has long aimed at the goal of the development of rapid and low cost methods for the detection of enteric viruses in water. Gene probes are small pieces of nucleic acid (either DNA or RNA) that hybridize to the complementary base pairs of specific genes and can be used to identify the genetic material of any organism. We report the development of cDNA probes against poliovirus and hepatitis A virus labeled with 32PdATP and 32PdCTP which is capable of detecting as little as one fg of nucleic acid or one tissue culture infective dose of poliovirus or one radio-immuno-focus unit of hepatitis A virus within 72 hours.",

author = "P. Gerba and B. Margolin and E. Trumper",

year = "1988",

language = "English (US)",

volume = "34",

pages = "518--519",

journal = "Zeitschrift fur die Gesamte Hygiene und Ihre Grenzgebiete",

issn = "0049-8610",

number = "9",

}

TY - JOUR

T1 - Enterovirus detection in water with gene probes

AU - Gerba, P.

AU - Margolin, B.

AU - Trumper, E.

PY - 1988

Y1 - 1988

N2 - Water virology has long aimed at the goal of the development of rapid and low cost methods for the detection of enteric viruses in water. Gene probes are small pieces of nucleic acid (either DNA or RNA) that hybridize to the complementary base pairs of specific genes and can be used to identify the genetic material of any organism. We report the development of cDNA probes against poliovirus and hepatitis A virus labeled with 32PdATP and 32PdCTP which is capable of detecting as little as one fg of nucleic acid or one tissue culture infective dose of poliovirus or one radio-immuno-focus unit of hepatitis A virus within 72 hours.

AB - Water virology has long aimed at the goal of the development of rapid and low cost methods for the detection of enteric viruses in water. Gene probes are small pieces of nucleic acid (either DNA or RNA) that hybridize to the complementary base pairs of specific genes and can be used to identify the genetic material of any organism. We report the development of cDNA probes against poliovirus and hepatitis A virus labeled with 32PdATP and 32PdCTP which is capable of detecting as little as one fg of nucleic acid or one tissue culture infective dose of poliovirus or one radio-immuno-focus unit of hepatitis A virus within 72 hours.

UR - http://www.scopus.com/inward/record.url?scp=0023734493&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023734493&partnerID=8YFLogxK

M3 - Article

C2 - 2850671

AN - SCOPUS:0023734493

SN - 0049-8610

VL - 34

SP - 518

EP - 519

JO - Zeitschrift fur die Gesamte Hygiene und Ihre Grenzgebiete

JF - Zeitschrift fur die Gesamte Hygiene und Ihre Grenzgebiete

IS - 9

ER -

Enterovirus detection in water with gene probes

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this