Enteral crude red kidney bean (Phaseolus vulgaris) lectin - Phytohemagglutinin - Induces maturational changes in the enterocyte membrane proteins of suckling rats

Danuta Kruszewska; Pawel Kiela; Åsa Ljungh; Kennedy H. Erlwanger; Björn R. Weström; Ann Linderoth; Stefan G. Pierzynowski

doi:10.1159/000071950

Enteral crude red kidney bean (Phaseolus vulgaris) lectin - Phytohemagglutinin - Induces maturational changes in the enterocyte membrane proteins of suckling rats

Danuta Kruszewska, Pawel Kiela, Åsa Ljungh, Kennedy H. Erlwanger, Björn R. Weström, Ann Linderoth, Stefan G. Pierzynowski

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

This study aimed to investigate the effect of enterally administered crude red kidney bean (Phaseolus vulgaris) lectin, PHA, on the expression of brush-border membrane vesicle (BBMV) proteins, in particular Na ⁺/H⁺ exchangers (NHEs), in the small intestine of suckling rats. Gavage of PHA to 14-day-old rats for 3 days resulted in altered protein/glycoprotein patterns as analyzed by SDS-PAGE. Immunoblots demonstrated the appearance of two 71- and 27-kD protein bands indicative for NHE3 - one of the NHE isoforms - and PHA, respectively. PHA treatment also resulted in an augmented uptake of ²²Na⁺ by the BBMV indicating an increase in NHE activity. Overall, the data suggests that enteral PHA exposure may induce maturational changes in enterocyte membrane proteins in young rats. In view of these findings, an investigation into the addition of PHA to infant formulas and weaning diets is warranted.

Original language	English (US)
Pages (from-to)	152-158
Number of pages	7
Journal	Biology of the Neonate
Volume	84
Issue number	2
DOIs	https://doi.org/10.1159/000071950
State	Published - 2003
Externally published	Yes

Keywords

Enterocyte maturation
Membrane glycosylation
Na/H exchanger
Phytohemagglutinin
Suckling rat

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Developmental Biology

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10.1159/000071950

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title = "Enteral crude red kidney bean (Phaseolus vulgaris) lectin - Phytohemagglutinin - Induces maturational changes in the enterocyte membrane proteins of suckling rats",

abstract = "This study aimed to investigate the effect of enterally administered crude red kidney bean (Phaseolus vulgaris) lectin, PHA, on the expression of brush-border membrane vesicle (BBMV) proteins, in particular Na +/H+ exchangers (NHEs), in the small intestine of suckling rats. Gavage of PHA to 14-day-old rats for 3 days resulted in altered protein/glycoprotein patterns as analyzed by SDS-PAGE. Immunoblots demonstrated the appearance of two 71- and 27-kD protein bands indicative for NHE3 - one of the NHE isoforms - and PHA, respectively. PHA treatment also resulted in an augmented uptake of 22Na+ by the BBMV indicating an increase in NHE activity. Overall, the data suggests that enteral PHA exposure may induce maturational changes in enterocyte membrane proteins in young rats. In view of these findings, an investigation into the addition of PHA to infant formulas and weaning diets is warranted.",

keywords = "Enterocyte maturation, Membrane glycosylation, Na/H exchanger, Phytohemagglutinin, Suckling rat",

author = "Danuta Kruszewska and Pawel Kiela and {\AA}sa Ljungh and Erlwanger, {Kennedy H.} and Westr{\"o}m, {Bj{\"o}rn R.} and Ann Linderoth and Pierzynowski, {Stefan G.}",

year = "2003",

doi = "10.1159/000071950",

language = "English (US)",

volume = "84",

pages = "152--158",

journal = "Biology of the Neonate",

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T1 - Enteral crude red kidney bean (Phaseolus vulgaris) lectin - Phytohemagglutinin - Induces maturational changes in the enterocyte membrane proteins of suckling rats

AU - Kruszewska, Danuta

AU - Kiela, Pawel

AU - Ljungh, Åsa

AU - Erlwanger, Kennedy H.

AU - Weström, Björn R.

AU - Linderoth, Ann

AU - Pierzynowski, Stefan G.

PY - 2003

Y1 - 2003

N2 - This study aimed to investigate the effect of enterally administered crude red kidney bean (Phaseolus vulgaris) lectin, PHA, on the expression of brush-border membrane vesicle (BBMV) proteins, in particular Na +/H+ exchangers (NHEs), in the small intestine of suckling rats. Gavage of PHA to 14-day-old rats for 3 days resulted in altered protein/glycoprotein patterns as analyzed by SDS-PAGE. Immunoblots demonstrated the appearance of two 71- and 27-kD protein bands indicative for NHE3 - one of the NHE isoforms - and PHA, respectively. PHA treatment also resulted in an augmented uptake of 22Na+ by the BBMV indicating an increase in NHE activity. Overall, the data suggests that enteral PHA exposure may induce maturational changes in enterocyte membrane proteins in young rats. In view of these findings, an investigation into the addition of PHA to infant formulas and weaning diets is warranted.

AB - This study aimed to investigate the effect of enterally administered crude red kidney bean (Phaseolus vulgaris) lectin, PHA, on the expression of brush-border membrane vesicle (BBMV) proteins, in particular Na +/H+ exchangers (NHEs), in the small intestine of suckling rats. Gavage of PHA to 14-day-old rats for 3 days resulted in altered protein/glycoprotein patterns as analyzed by SDS-PAGE. Immunoblots demonstrated the appearance of two 71- and 27-kD protein bands indicative for NHE3 - one of the NHE isoforms - and PHA, respectively. PHA treatment also resulted in an augmented uptake of 22Na+ by the BBMV indicating an increase in NHE activity. Overall, the data suggests that enteral PHA exposure may induce maturational changes in enterocyte membrane proteins in young rats. In view of these findings, an investigation into the addition of PHA to infant formulas and weaning diets is warranted.

KW - Enterocyte maturation

KW - Membrane glycosylation

KW - Na/H exchanger

KW - Phytohemagglutinin

KW - Suckling rat

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Enteral crude red kidney bean (Phaseolus vulgaris) lectin - Phytohemagglutinin - Induces maturational changes in the enterocyte membrane proteins of suckling rats

Abstract

Keywords

ASJC Scopus subject areas

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