Enhanced capacitative calcium entry and sarcoplasmic-reticulum calcium storage capacity with advanced age in murine mesenteric arterial smooth muscle cells

Ravi Goyal, Jeff E. Angermann, Olga Ostrovskaya, John N. Buchholz, Gregory D. Smith, Sean M. Wilson

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Intracellular Ca2+ signaling is important to perfusion pressure related arterial reactivity and to vascular disorders including hypertension, angina and ischemic stroke. We have recently shown that advancing-age leads to calcium signaling adaptations in mesenteric arterial myocytes from C57 BL/6 mice [Corsso, C.D., Ostrovskaya, O., McAllister, C.E., Murray, K., Hatton, W.J., Gurney, A.M., Spencer, N.J., Wilson, S.M., 2006. Effects of aging on Ca(2+) signaling in murine mesenteric arterial smooth muscle cells. Mech. Ageing Dev. 127, 315-323)] which may contribute to decrements in perfusion pressure related arterial contractility others have shown occur. Even still, the mechanisms underlying the changes in Ca2+ signaling and arterial reactivity are unresolved. Ca2+ transport and storage capabilities are thought to contribute to age-related Ca2+ signaling dysfunctions in other cell types. The present studies were therefore designed to test the hypothesis that cytosolic and compartmental Ca2+ homeostasis in mesenteric arterial myocytes changes with advanced age. The hypothesis was tested by performing digitalized fluorescence microscopy on mesenteric arterial myocytes isolated from 5- to 6-month and 29- to 30-month-old C57Bl/6 mice. The data provide evidence that with advanced age capacitative Ca2+ entry and sarcoplasmic reticulum Ca2+ storage are increased although sarcoplasmic reticulum Ca2+ uptake and plasma membrane Ca2+ extrusion are unaltered. Overall, the studies begin to resolve the mechanisms associated with age-related alterations in mesenteric arterial smooth muscle Ca2+ signaling and their physiological consequences.

Original languageEnglish (US)
Pages (from-to)201-207
Number of pages7
JournalExperimental Gerontology
Volume44
Issue number3
DOIs
StatePublished - Mar 2009
Externally publishedYes

Keywords

  • PMCA
  • SERCA
  • SOC
  • fura-2

ASJC Scopus subject areas

  • Biochemistry
  • Aging
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Cell Biology

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