Engineering quadrupole magnetic flow sorting for the isolation of pancreatic islets

David J. Kennedy; Paul Todd; Sam Logan; Matthew Becker; Klearchos K. Papas; Lee R. Moore

doi:10.1016/j.jmmm.2006.10.1160

Engineering quadrupole magnetic flow sorting for the isolation of pancreatic islets

David J. Kennedy, Paul Todd, Sam Logan, Matthew Becker, Klearchos K. Papas, Lee R. Moore

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Quadrupole magnetic flow sorting (QMS) is being adapted from the separation of suspensions of single cells (<15 μm) to the isolation of pancreatic islets (150-350 μm) for transplant. To achieve this goal, the critical QMS components have been modeled and engineered to optimize the separation process. A flow channel has been designed, manufactured, and tested. The quadrupole magnet assembly has been designed and verified by finite element analysis. Pumps have been selected and verified by test. Test data generated from the pumps and flow channel demonstrate that the fabricated channel and peristaltic pumps fulfill the requirements of successful QMS separation.

Original language	English (US)
Pages (from-to)	388-395
Number of pages	8
Journal	Journal of Magnetism and Magnetic Materials
Volume	311
Issue number	1 SPEC. ISS.
DOIs	https://doi.org/10.1016/j.jmmm.2006.10.1160
State	Published - Apr 2007
Externally published	Yes

Keywords

Cell separation
Engineering
Magnetic separation
Pancreatic islets
Quadrupole separator
Type 1 diabetes

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics

Access to Document

10.1016/j.jmmm.2006.10.1160

Cite this

@article{63b11363f12d4e5f9354c24ad214dbad,

title = "Engineering quadrupole magnetic flow sorting for the isolation of pancreatic islets",

abstract = "Quadrupole magnetic flow sorting (QMS) is being adapted from the separation of suspensions of single cells (<15 μm) to the isolation of pancreatic islets (150-350 μm) for transplant. To achieve this goal, the critical QMS components have been modeled and engineered to optimize the separation process. A flow channel has been designed, manufactured, and tested. The quadrupole magnet assembly has been designed and verified by finite element analysis. Pumps have been selected and verified by test. Test data generated from the pumps and flow channel demonstrate that the fabricated channel and peristaltic pumps fulfill the requirements of successful QMS separation.",

keywords = "Cell separation, Engineering, Magnetic separation, Pancreatic islets, Quadrupole separator, Type 1 diabetes",

author = "Kennedy, {David J.} and Paul Todd and Sam Logan and Matthew Becker and Papas, {Klearchos K.} and Moore, {Lee R.}",

note = "Funding Information: The authors thank the National Institutes of Health (1 R43 DK072647-01) and the Indiana 21st Century Research and Technology Fund for their financial support. The authors also thank Dr. Bernhard Hering and his staff at the Diabetes Institute for Immunology and Transplantation, Dr. Jeff Chalmers and Mr. Rustin Shenkman at The Ohio State University, and Dr. Maciej Zborowski at the Cleveland Clinic Foundation for their technical support.",

year = "2007",

month = apr,

doi = "10.1016/j.jmmm.2006.10.1160",

language = "English (US)",

volume = "311",

pages = "388--395",

journal = "Journal of Magnetism and Magnetic Materials",

issn = "0304-8853",

publisher = "Elsevier B.V.",

number = "1 SPEC. ISS.",

}

TY - JOUR

T1 - Engineering quadrupole magnetic flow sorting for the isolation of pancreatic islets

AU - Kennedy, David J.

AU - Todd, Paul

AU - Logan, Sam

AU - Becker, Matthew

AU - Papas, Klearchos K.

AU - Moore, Lee R.

N1 - Funding Information: The authors thank the National Institutes of Health (1 R43 DK072647-01) and the Indiana 21st Century Research and Technology Fund for their financial support. The authors also thank Dr. Bernhard Hering and his staff at the Diabetes Institute for Immunology and Transplantation, Dr. Jeff Chalmers and Mr. Rustin Shenkman at The Ohio State University, and Dr. Maciej Zborowski at the Cleveland Clinic Foundation for their technical support.

PY - 2007/4

Y1 - 2007/4

N2 - Quadrupole magnetic flow sorting (QMS) is being adapted from the separation of suspensions of single cells (<15 μm) to the isolation of pancreatic islets (150-350 μm) for transplant. To achieve this goal, the critical QMS components have been modeled and engineered to optimize the separation process. A flow channel has been designed, manufactured, and tested. The quadrupole magnet assembly has been designed and verified by finite element analysis. Pumps have been selected and verified by test. Test data generated from the pumps and flow channel demonstrate that the fabricated channel and peristaltic pumps fulfill the requirements of successful QMS separation.

AB - Quadrupole magnetic flow sorting (QMS) is being adapted from the separation of suspensions of single cells (<15 μm) to the isolation of pancreatic islets (150-350 μm) for transplant. To achieve this goal, the critical QMS components have been modeled and engineered to optimize the separation process. A flow channel has been designed, manufactured, and tested. The quadrupole magnet assembly has been designed and verified by finite element analysis. Pumps have been selected and verified by test. Test data generated from the pumps and flow channel demonstrate that the fabricated channel and peristaltic pumps fulfill the requirements of successful QMS separation.

KW - Cell separation

KW - Engineering

KW - Magnetic separation

KW - Pancreatic islets

KW - Quadrupole separator

KW - Type 1 diabetes

UR - http://www.scopus.com/inward/record.url?scp=33947120636&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947120636&partnerID=8YFLogxK

U2 - 10.1016/j.jmmm.2006.10.1160

DO - 10.1016/j.jmmm.2006.10.1160

M3 - Article

AN - SCOPUS:33947120636

SN - 0304-8853

VL - 311

SP - 388

EP - 395

JO - Journal of Magnetism and Magnetic Materials

JF - Journal of Magnetism and Magnetic Materials

IS - 1 SPEC. ISS.

ER -

Engineering quadrupole magnetic flow sorting for the isolation of pancreatic islets

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this