Engineering Iron Responses in Mammalian Cells by Signal-Induced Protein Proximity

Guihua Zeng; Huanqiu Li; Yongyi Wei; Weimin Xuan; Roushu Zhang; Larisa E. Breden; Wei Wang; Fu Sen Liang

doi:10.1021/acssynbio.6b00255

Engineering Iron Responses in Mammalian Cells by Signal-Induced Protein Proximity

Guihua Zeng, Huanqiu Li, Yongyi Wei, Weimin Xuan, Roushu Zhang, Larisa E. Breden, Wei Wang, Fu Sen Liang

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

A new synthetic biology engineering strategy integrating chemical reactivity sensing and small molecule induced protein dimerization has been developed to generate artificial Fe²⁺ signaling circuitry to control tailored cellular events in mammalian cells. The dual function probe ABA-FE18 (Fe²⁺-sensing and protein dimerization) derived from ABA was developed and used to control gene activation, signal transduction, and cytoskeletal remodeling in response to Fe²⁺. This technology was utilized to design signal circuitry incorporating "AND" and "OR" biologic gates that enables mammalian cells to translate different combinations of Fe²⁺ and H₂O₂ signals into predefined biological outputs.

Original language	English (US)
Pages (from-to)	921-927
Number of pages	7
Journal	ACS Synthetic Biology
Volume	6
Issue number	6
DOIs	https://doi.org/10.1021/acssynbio.6b00255
State	Published - Jun 16 2017
Externally published	Yes

Keywords

abscisic acid
biologic gate
biosensor
cell signaling
chemically induced proximity
labile iron

ASJC Scopus subject areas

Biomedical Engineering
Biochemistry, Genetics and Molecular Biology (miscellaneous)

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10.1021/acssynbio.6b00255

Cite this

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abstract = "A new synthetic biology engineering strategy integrating chemical reactivity sensing and small molecule induced protein dimerization has been developed to generate artificial Fe2+ signaling circuitry to control tailored cellular events in mammalian cells. The dual function probe ABA-FE18 (Fe2+-sensing and protein dimerization) derived from ABA was developed and used to control gene activation, signal transduction, and cytoskeletal remodeling in response to Fe2+. This technology was utilized to design signal circuitry incorporating {"}AND{"} and {"}OR{"} biologic gates that enables mammalian cells to translate different combinations of Fe2+ and H2O2 signals into predefined biological outputs.",

keywords = "abscisic acid, biologic gate, biosensor, cell signaling, chemically induced proximity, labile iron",

author = "Guihua Zeng and Huanqiu Li and Yongyi Wei and Weimin Xuan and Roushu Zhang and Breden, {Larisa E.} and Wei Wang and Liang, {Fu Sen}",

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T1 - Engineering Iron Responses in Mammalian Cells by Signal-Induced Protein Proximity

AU - Zeng, Guihua

AU - Li, Huanqiu

AU - Wei, Yongyi

AU - Xuan, Weimin

AU - Zhang, Roushu

AU - Breden, Larisa E.

AU - Wang, Wei

AU - Liang, Fu Sen

PY - 2017/6/16

Y1 - 2017/6/16

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AB - A new synthetic biology engineering strategy integrating chemical reactivity sensing and small molecule induced protein dimerization has been developed to generate artificial Fe2+ signaling circuitry to control tailored cellular events in mammalian cells. The dual function probe ABA-FE18 (Fe2+-sensing and protein dimerization) derived from ABA was developed and used to control gene activation, signal transduction, and cytoskeletal remodeling in response to Fe2+. This technology was utilized to design signal circuitry incorporating "AND" and "OR" biologic gates that enables mammalian cells to translate different combinations of Fe2+ and H2O2 signals into predefined biological outputs.

KW - abscisic acid

KW - biologic gate

KW - biosensor

KW - cell signaling

KW - chemically induced proximity

KW - labile iron

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Engineering Iron Responses in Mammalian Cells by Signal-Induced Protein Proximity

Abstract

Keywords

ASJC Scopus subject areas

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