Engineering Iron Responses in Mammalian Cells by Signal-Induced Protein Proximity

Guihua Zeng, Huanqiu Li, Yongyi Wei, Weimin Xuan, Roushu Zhang, Larisa E. Breden, Wei Wang, Fu Sen Liang

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A new synthetic biology engineering strategy integrating chemical reactivity sensing and small molecule induced protein dimerization has been developed to generate artificial Fe2+ signaling circuitry to control tailored cellular events in mammalian cells. The dual function probe ABA-FE18 (Fe2+-sensing and protein dimerization) derived from ABA was developed and used to control gene activation, signal transduction, and cytoskeletal remodeling in response to Fe2+. This technology was utilized to design signal circuitry incorporating "AND" and "OR" biologic gates that enables mammalian cells to translate different combinations of Fe2+ and H2O2 signals into predefined biological outputs.

Original languageEnglish (US)
Pages (from-to)921-927
Number of pages7
JournalACS Synthetic Biology
Volume6
Issue number6
DOIs
StatePublished - Jun 16 2017
Externally publishedYes

Keywords

  • abscisic acid
  • biologic gate
  • biosensor
  • cell signaling
  • chemically induced proximity
  • labile iron

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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