Energetic metabolism of glucose, mannose and galactose in glucose-starved rat insulinoma cells anchored on microcarrier beads. A phosphorus-31 NMR study

M. Kriat; J. Fantini; J. Vion-Dury; S. Confort-Gouny; J. P. Galons; P. J. Cozzone

doi:10.1016/0300-9084(92)90079-T

Energetic metabolism of glucose, mannose and galactose in glucose-starved rat insulinoma cells anchored on microcarrier beads. A phosphorus-31 NMR study

M. Kriat, J. Fantini, J. Vion-Dury, S. Confort-Gouny, J. P. Galons, P. J. Cozzone

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Insulin-secreting cells (RINm5F) have successfully been grown on a large scale on poly-l-lysine coated-polystyrene microcarriers, providing a high cell number in a restricted volume under conditions that respect the metabolic integrity of these anchorage-dependent cells. The energetic metabolism of the perfused cells hae been followed non-invasively by phosphorus-31 nuclear magnetic resonance spectroscopy. Glucose starvation induced a rapid decrease in nucleoside triphosphates (mainly ATP) pools, correlated with an increase in Pi level. The initial ATP level was rapidly recovered when the cells were refed with glucose or wth mannose, but not with galactose, even after 2 h of perfusion. These differential effects of hexoses on energetic metabolism might be related to their various insulin-release actions on tumor islet cells.

Original language	English (US)
Pages (from-to)	949-955
Number of pages	7
Journal	Biochimie
Volume	74
Issue number	9-10
DOIs	https://doi.org/10.1016/0300-9084(92)90079-T
State	Published - 1992
Externally published	Yes

Keywords

P NMR spectroscopy/ RINm5F cells/ microcarriers culture/ hexose metabolism

ASJC Scopus subject areas

Biochemistry

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10.1016/0300-9084(92)90079-T

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title = "Energetic metabolism of glucose, mannose and galactose in glucose-starved rat insulinoma cells anchored on microcarrier beads. A phosphorus-31 NMR study",

abstract = "Insulin-secreting cells (RINm5F) have successfully been grown on a large scale on poly-l-lysine coated-polystyrene microcarriers, providing a high cell number in a restricted volume under conditions that respect the metabolic integrity of these anchorage-dependent cells. The energetic metabolism of the perfused cells hae been followed non-invasively by phosphorus-31 nuclear magnetic resonance spectroscopy. Glucose starvation induced a rapid decrease in nucleoside triphosphates (mainly ATP) pools, correlated with an increase in Pi level. The initial ATP level was rapidly recovered when the cells were refed with glucose or wth mannose, but not with galactose, even after 2 h of perfusion. These differential effects of hexoses on energetic metabolism might be related to their various insulin-release actions on tumor islet cells.",

keywords = "P NMR spectroscopy/ RINm5F cells/ microcarriers culture/ hexose metabolism",

author = "M. Kriat and J. Fantini and J. Vion-Dury and S. Confort-Gouny and Galons, {J. P.} and Cozzone, {P. J.}",

year = "1992",

doi = "10.1016/0300-9084(92)90079-T",

language = "English (US)",

volume = "74",

pages = "949--955",

journal = "Biochimie",

issn = "0300-9084",

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T1 - Energetic metabolism of glucose, mannose and galactose in glucose-starved rat insulinoma cells anchored on microcarrier beads. A phosphorus-31 NMR study

AU - Kriat, M.

AU - Fantini, J.

AU - Vion-Dury, J.

AU - Confort-Gouny, S.

AU - Galons, J. P.

AU - Cozzone, P. J.

PY - 1992

Y1 - 1992

N2 - Insulin-secreting cells (RINm5F) have successfully been grown on a large scale on poly-l-lysine coated-polystyrene microcarriers, providing a high cell number in a restricted volume under conditions that respect the metabolic integrity of these anchorage-dependent cells. The energetic metabolism of the perfused cells hae been followed non-invasively by phosphorus-31 nuclear magnetic resonance spectroscopy. Glucose starvation induced a rapid decrease in nucleoside triphosphates (mainly ATP) pools, correlated with an increase in Pi level. The initial ATP level was rapidly recovered when the cells were refed with glucose or wth mannose, but not with galactose, even after 2 h of perfusion. These differential effects of hexoses on energetic metabolism might be related to their various insulin-release actions on tumor islet cells.

AB - Insulin-secreting cells (RINm5F) have successfully been grown on a large scale on poly-l-lysine coated-polystyrene microcarriers, providing a high cell number in a restricted volume under conditions that respect the metabolic integrity of these anchorage-dependent cells. The energetic metabolism of the perfused cells hae been followed non-invasively by phosphorus-31 nuclear magnetic resonance spectroscopy. Glucose starvation induced a rapid decrease in nucleoside triphosphates (mainly ATP) pools, correlated with an increase in Pi level. The initial ATP level was rapidly recovered when the cells were refed with glucose or wth mannose, but not with galactose, even after 2 h of perfusion. These differential effects of hexoses on energetic metabolism might be related to their various insulin-release actions on tumor islet cells.

KW - P NMR spectroscopy/ RINm5F cells/ microcarriers culture/ hexose metabolism

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U2 - 10.1016/0300-9084(92)90079-T

DO - 10.1016/0300-9084(92)90079-T

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AN - SCOPUS:0026497040

SN - 0300-9084

VL - 74

SP - 949

EP - 955

JO - Biochimie

JF - Biochimie

IS - 9-10

ER -

Energetic metabolism of glucose, mannose and galactose in glucose-starved rat insulinoma cells anchored on microcarrier beads. A phosphorus-31 NMR study

Abstract

Keywords

ASJC Scopus subject areas

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