Endothelin-1 mRNA in glomerular and epithelial cells of kidney

Min Chen, Karyn Todd-Turla, Wei Hua Wang, Xinan Cao, Ann Smart, Frank C. Brosius, Paul D. Killen, Joan A. Keiser, Josie P. Briggs, Jürgen Schnermann

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

To examine the question of the tubular lo-calization of renal endothelin-1 (ET-1) mRNA, cDNA generated by reverse transcription of isolated rat tubule RNA was amplified by polymerase chain reaction using rat ET-1-specific oligonucleotides. Product identity was determined by restriction enzyme digestion or direct product sequencing. ET-1 mRNA was found to increase in renal tissue in a corticomedullary direction. High levels of ET-1 mRNA were found in dissected glomeruli and in juxtaglomerular cells in short-term primary culture. Among tubule segments, ET-1 mRNA was most abundant in inner medullary collecting ducts (IMCD), but products were also found with cDNA derived from proximal convoluted and straight tubules, thick ascending limbs, and outer medullary collecting ducts. In kidneys of untreated, homozygous Brattleboro rats, the increase of ET-1 mRNA along the corticomedullary axis as well as the preponderance of tubular ET-1 mRNA in IMCD was not observed. Our data show that ET-1 mRNA is present in all nephron segments studied and that its expression may be dependent on the functional state of the kidney. Our results are consistent with the proposal that ET-1 modifies tubular function in an autocrine or paracrine fashion.

Original languageEnglish (US)
Pages (from-to)F542-F550
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume265
Issue number4 34-4
StatePublished - 1993
Externally publishedYes

Keywords

  • Isolated granular cells
  • Kidney tubules
  • Rat kidney
  • Reverse transcription-polymerase chain reaction: Brattleboro rats

ASJC Scopus subject areas

  • Physiology

Fingerprint

Dive into the research topics of 'Endothelin-1 mRNA in glomerular and epithelial cells of kidney'. Together they form a unique fingerprint.

Cite this