TY - JOUR
T1 - Endogenous levels of β-carotene in human buccal mucosa cells by reversed-phase high-performance liquid chromatography
AU - Newcomb, Stephen A.
AU - Culling-Berglund, Alison J.
AU - Davis, Thomas P.
N1 - Funding Information:
The authors wish to thank Dr. David Alberts, Dr. P.M. Plezia and Susan Sayers of the Arizona Cancer Center for providing the samples used in the kinetic study. This work was supported by N.I.H. Grants CA27502, CA44869 and DK36289 to Thomas P. Davis.
PY - 1990
Y1 - 1990
N2 - We have developed a reversed-phase high-performance liquid chromatographic assay for the measurement of low nanogram levels of β-carotene in a single sample of human buccal mucosa cells. The method includes a simple sonification step for cell disruption and release of the compounds into the supernatant. The limits of detection were 0.02, 0.02 and 0.07 ng/mg of protein for β-carotene, retinol and retinol palmitate, respectively. Two patient populations were analysed. Average endogenous levels for β-carotene normalized to protein were 0.25 ng/mg of protein (range 0.04-1.9 ng/mg, twelve patients). No evidence of endogenous retinol or retinol palmitate could be detected in the human samples. An oral dosing study of four normal individuals showed a wide variation of β-carotene uptake. This rapid and sensitive method will enable investigators to use the non-invasive technique of buccal mucosa cell harvesting to determine cellular depot levels of β-carotene in various patient populations.
AB - We have developed a reversed-phase high-performance liquid chromatographic assay for the measurement of low nanogram levels of β-carotene in a single sample of human buccal mucosa cells. The method includes a simple sonification step for cell disruption and release of the compounds into the supernatant. The limits of detection were 0.02, 0.02 and 0.07 ng/mg of protein for β-carotene, retinol and retinol palmitate, respectively. Two patient populations were analysed. Average endogenous levels for β-carotene normalized to protein were 0.25 ng/mg of protein (range 0.04-1.9 ng/mg, twelve patients). No evidence of endogenous retinol or retinol palmitate could be detected in the human samples. An oral dosing study of four normal individuals showed a wide variation of β-carotene uptake. This rapid and sensitive method will enable investigators to use the non-invasive technique of buccal mucosa cell harvesting to determine cellular depot levels of β-carotene in various patient populations.
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U2 - 10.1016/S0378-4347(00)82482-2
DO - 10.1016/S0378-4347(00)82482-2
M3 - Article
C2 - 2341545
AN - SCOPUS:0025255578
SN - 0378-4347
VL - 526
SP - 47
EP - 58
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - C
ER -