TY - JOUR
T1 - Embryonic stem cells as a model for cardiogenesis
AU - Robbins, Jeffrey
AU - Doetschman, Thomas
AU - Jones, W. Keith
AU - Sánchez, Alejandro
N1 - Funding Information:
This work was supported by grants from the National Institutes of Health (HL 41496 to J .R and TD . ; HL 22619 to J.R .), the American Heart Association (J .R. and T.D .), and a Biomedical Re- search Science Grant (TD .) . J .R. is an Established Investigator of the American Heart Association.
PY - 1992
Y1 - 1992
N2 - Embryonic stem (ES) cells are derived from the inner cell mass of mouse blastocysts. These cells, when placed upon a suitable fibroblast feeder layer, continue to proliferate without overt differentiation and remain totipotent. Cells in this state are competent for gene targeting via homologous recombination. Hence, they hold the possibility of developing defined animal models of human cardiovascular disease. When removed from the feeder layer, ES cells undergo differentiation and development into large, multicellular structures, termed embryoid bodies (EBs). Morphologic, biochemical, and molecular genetic analyses indicate that during EB development some early aspects of cardiogenesis are recapitulated. Thus, EB development in culture is useful for studying certain early cardiogenic events.
AB - Embryonic stem (ES) cells are derived from the inner cell mass of mouse blastocysts. These cells, when placed upon a suitable fibroblast feeder layer, continue to proliferate without overt differentiation and remain totipotent. Cells in this state are competent for gene targeting via homologous recombination. Hence, they hold the possibility of developing defined animal models of human cardiovascular disease. When removed from the feeder layer, ES cells undergo differentiation and development into large, multicellular structures, termed embryoid bodies (EBs). Morphologic, biochemical, and molecular genetic analyses indicate that during EB development some early aspects of cardiogenesis are recapitulated. Thus, EB development in culture is useful for studying certain early cardiogenic events.
UR - http://www.scopus.com/inward/record.url?scp=0026603780&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026603780&partnerID=8YFLogxK
U2 - 10.1016/1050-1738(92)90003-B
DO - 10.1016/1050-1738(92)90003-B
M3 - Short survey
C2 - 21239273
AN - SCOPUS:0026603780
SN - 1050-1738
VL - 2
SP - 44
EP - 50
JO - Trends in Cardiovascular Medicine
JF - Trends in Cardiovascular Medicine
IS - 2
ER -