@article{7a1be307c05a41baa05714bf5f1bf060,
title = "EMB30 is essential for normal cell division, cell expansion, and cell adhesion in Arabidopsis and encodes a protein that has similarity to Sec7",
abstract = "The EMB30 gene is involved in apical-basal pattern formation in the Arabidopsis embryo. Mutations in this locus produce mutants with a wide range of seedling phenotypes, but all of the mutants lack a root and a true hypocotyl. We have cloned the EMB30 gene, and it encodes a protein that has similarity to the yeast Sec7 protein and to two other open reading frames identified in clones from humans and C. elegans. We refer to the region of similarity among these four sequences as the Sec7 domain. The emb30-1 allele has a mutation in the Sec7 domain that alters a residue conserved in all four of these sequences, suggesting that this domain may be important for EMB30 function. Molecular data and microscopy studies of emb30 seedlings presented here indicate that EMB30 affects cell division, elongation, and adhesion and functions in seedling and adult plants as well as during embryogenic pattern formation.",
author = "Shevell, {Diane E.} and Leu, {Wei Ming} and Gillmor, {C. Stewart} and Guixian Xia and Feldmann, {Kenneth A.} and Chua, {Nam Hai}",
note = "Funding Information: We thank Pablo Scolnick and the DuPont de Nemours company for allowing us to screen the insertion lines and Kathy Barton, Philip Ben-fey, Shirley Coomber, and Liam Dolan for help with screening the lines. We thank D. Meinke for suggesting the cross to embdO-l and for sending emb30-I, emb30-2, and wild-type Columbia seed, and Kathy Barton for sending us seedling mutants that we confirmed to be emb30 mutants (emb30-5 and emb30-6). We are also grateful to Helen Shio of the Electron Microscopy Service for expert technical assistance with the microscopy analysis. We thank P. Benfey for help with plant techniques and useful discussions. We also thank Gunther Neuhaus for fruitful discussions and for comments on the manuscript, and Diana Horvath, Robert McGrath, and Raphael Mayer for careful reading of the manuscript. D. E. S. was supported by a fellowship from the National Institutes of Health, and G. X. was supported by the Institute of Molecular and Cell Biology, Singapore. This work was supported in part by the Human Frontier Science Program.",
year = "1994",
month = jul,
day = "1",
doi = "10.1016/0092-8674(94)90444-8",
language = "English (US)",
volume = "77",
pages = "1051--1062",
journal = "Cell",
issn = "0092-8674",
publisher = "Elsevier B.V.",
number = "7",
}