Elicitation of anti-H-2 cytotoxic T lymphocytes with antigen-modified H-2 negative stimulator cells

By a newly developed technique, we have modified H-2(k)-negative cells in such a way as to render them capable of elicitation of anti-H-2 CTL. Purified H-2K(k) and/or partially purified Ia(k) glycoproteins were incorporated into lipid vesicles that contained the hemagglutinin-neuraminidase (HN) glycoprotein and the fusion (F) glycoprotein of Sendai virus. These lipid vesicles were incubated with H-2-negative tumor cells (R1^-) in a manner that resulted in rendering these cells capable of eliciting anti-H-2K(k) CTL. The pressure of Ia(k) on the surface of the modified stimulator cell had a significant effect on the proportion of anti-H-2K(k) CTL possessing a particular Lyt phenotype. Primary effector cells elicited by an H-2K(k)-Ia(k) difference were primarily Lyt 1^-, 2⁺, whereas primary anti-H-2K(k) CTL elicited by an H-2K(k) difference alone were predominantly Lyt 1⁺, 2⁺.