TY - JOUR
T1 - Elevated amniotic fluid nitric oxide levels of metabolites in pregnant women with intraamniotic infection
AU - Hsu, C. D.
AU - Aversa, K.
AU - Barsheertf, H.
AU - Meaddough, E.
AU - Hong', S. F.
AU - Lef, I. S.
AU - Jones, B. C.
AU - Copel, J. A.
PY - 1997
Y1 - 1997
N2 - OBJECTIVE: Bacterial infection induces macrophage and neutrophil activation to produce nitric oxide. Nitric oxide is unstable and breaks down rapidly to nitrate and nitrite. We compared the amniotic fluid nitric oxide metabolites in pregnant women with and without intraamniotic infection (IAI). STUDY DESIGN: Amniocentesis was performed on 7H pregnant women with preterm contractions, labor, or rupture of membranes. Fifteen patients had IAI and 61 did not. IAI was defined as the presence of a positive amniotic fluid culture. Amniotic fluid gram stain, glucose, neutrophil counts, leukocyte esterase activity, creatinine, pH, specific gravity were performed. Endogenous nitrite was determined using Griess reagent. Total nitrate and nitrite (NOx) were measured after reduction of nitrate to nitrite with aspergillus nitrate redtictase. Two-tailed t test, contingency table methods, linear regression and correlation were used for statistical analyses. RESULTS: Amniotic fluid levels of NOx and endogenous nitrite were significantly higher in pregnant women with IAI than those without IAI (2.73 ±0.46 vs. 1.78 ±0.08 jjimol/mg creatinine, p = 0.001, and 0.70 ± 0.13 vs. 0.37 ±0.03 fxmol/mg creatinine, p - 0.0009, respectively). IAI had a significantly higher incidence of amniotic fluid gram stain, leukocyte esterase activity, leukocyte counts, and a significantly lower amniotic fluid glucose than without IAI. Amniotic fluid NOx and endogenous nitrite were also significantly correlated with leukocue esterase activity, gram stain, leukocyte counts positively, and glucose negatively. CONCLUSIONS: Our data provide important information for better understanding the role of amniotic fluid nitric oxide in the pathogenesis of IAI. Measurements of amniotic fluid nitric oxide metabolite may be pari of a panel of tests that can be used to detect IAI.
AB - OBJECTIVE: Bacterial infection induces macrophage and neutrophil activation to produce nitric oxide. Nitric oxide is unstable and breaks down rapidly to nitrate and nitrite. We compared the amniotic fluid nitric oxide metabolites in pregnant women with and without intraamniotic infection (IAI). STUDY DESIGN: Amniocentesis was performed on 7H pregnant women with preterm contractions, labor, or rupture of membranes. Fifteen patients had IAI and 61 did not. IAI was defined as the presence of a positive amniotic fluid culture. Amniotic fluid gram stain, glucose, neutrophil counts, leukocyte esterase activity, creatinine, pH, specific gravity were performed. Endogenous nitrite was determined using Griess reagent. Total nitrate and nitrite (NOx) were measured after reduction of nitrate to nitrite with aspergillus nitrate redtictase. Two-tailed t test, contingency table methods, linear regression and correlation were used for statistical analyses. RESULTS: Amniotic fluid levels of NOx and endogenous nitrite were significantly higher in pregnant women with IAI than those without IAI (2.73 ±0.46 vs. 1.78 ±0.08 jjimol/mg creatinine, p = 0.001, and 0.70 ± 0.13 vs. 0.37 ±0.03 fxmol/mg creatinine, p - 0.0009, respectively). IAI had a significantly higher incidence of amniotic fluid gram stain, leukocyte esterase activity, leukocyte counts, and a significantly lower amniotic fluid glucose than without IAI. Amniotic fluid NOx and endogenous nitrite were also significantly correlated with leukocue esterase activity, gram stain, leukocyte counts positively, and glucose negatively. CONCLUSIONS: Our data provide important information for better understanding the role of amniotic fluid nitric oxide in the pathogenesis of IAI. Measurements of amniotic fluid nitric oxide metabolite may be pari of a panel of tests that can be used to detect IAI.
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M3 - Article
AN - SCOPUS:33748603464
SN - 0001-5563
VL - 176
SP - S40
JO - Acta Diabetologica Latina
JF - Acta Diabetologica Latina
IS - 1 PART II
ER -