Electron microscopy of rhamnolipid (biosurfactant) morphology: Effects of pH, cadmium, and octadecane

Jill T. Champion, John C. Gilkey, Henry Lamparski, Jennifer Retterer, Raina M. Miller

Research output: Contribution to journalArticlepeer-review

130 Scopus citations


It was previously reported that rhamnolipid bisurfactant from Pseudomonas aeruginosa ATCC 9027 can be used to remediate contaminated sites. Since the effective use of rhamnolipid require an understanding of its morphology as well as the effects of pH and organic and inorganic contaminants on that morphology, cryo-transmission electron microscopy was used to examine the morphology of vitrified, frozen hydrated suspensions of rhamnolipid over a pH range of 5.5 to 8.0. The effect of a model alkane, octadecane and a model heavy metal cadmium on the morphology of the rhamnolipid was also determined. Results obtained are found to be satisfactory.

Original languageEnglish (US)
Pages (from-to)569-574
Number of pages6
JournalJournal of Colloid And Interface Science
Issue number2
StatePublished - Mar 15 1995


  • Bioremediation
  • Biosurfactant
  • Rhamnolipid

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry


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