Efficient transplantation via antibody-based clearance of hematopoietic stem cell niches

Agnieszka Czechowicz, Daniel Kraft, Irving L. Weissman, Deepta Bhattacharya

Research output: Contribution to journalArticlepeer-review

290 Scopus citations

Abstract

Upon intravenous transplantation, hematopoietic stem cells (HSCs) can home to specialized niches, yet most HSCs fail to engraft unless recipients are subjected to toxic preconditioning. We provide evidence that, aside from immune barriers, donor HSC engraftment is restricted by occupancy of appropriate niches by host HSCs. Administration of ACK2, an antibody that blocks c-kit function, led to the transient removal of >98% of endogenous HSCs in immunodeficient mice. Subsequent transplantation of these mice with donor HSCs led to chimerism levels of up to 90%. Extrapolation of these methods to humans may enable mild but effective conditioning regimens for transplantation.

Original languageEnglish (US)
Pages (from-to)1296-1299
Number of pages4
JournalScience
Volume318
Issue number5854
DOIs
StatePublished - Nov 23 2007
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'Efficient transplantation via antibody-based clearance of hematopoietic stem cell niches'. Together they form a unique fingerprint.

Cite this