TY - JOUR
T1 - Effects of the polyamine analogs BE-3-7-3, 3-8-3, and BE-3-8-3 on human brain tumor cell growth and survival
AU - Basu, H. S.
AU - Pellarin, M.
AU - Feuerstein, B. G.
AU - Deen, D. F.
AU - Marton, L. J.
PY - 1993
Y1 - 1993
N2 - Based on computer modeling, physicochemical studies of spermine-DNA interactions, and cell culture experiments, we hypothesized that polyamine analogs with hydrocarbon chain lengths differing from natural polyamines and a stronger affinity for nucleic acids than spermine should have a cellular antiproliferative effect. We tested three spermine analogs with long hydrocarbon chains, 1,16-diamino-4,12-diazahexadecane (3-8-3), 1,16-bis(ethyl)amino-4,12-diazahexadecane (BE-3-8-3), and 1,15-bis(ethyl)amino-4,11-diazapentadecane (BE-3-7-3) in human brain tumor cell lines U-251 MG, SF-126, and SF-188. Analog concentrations ≤ 5 μM inhibited growth and colony-forming efficiency in each cell line by treatment day 5, with significant decreases in putrescine and spermidine, but not spermine, levels. These findings suggest that potentially cytotoxic polyamine analogs can be specified on the basis of their hydrocarbon chain length and DNA affinity.
AB - Based on computer modeling, physicochemical studies of spermine-DNA interactions, and cell culture experiments, we hypothesized that polyamine analogs with hydrocarbon chain lengths differing from natural polyamines and a stronger affinity for nucleic acids than spermine should have a cellular antiproliferative effect. We tested three spermine analogs with long hydrocarbon chains, 1,16-diamino-4,12-diazahexadecane (3-8-3), 1,16-bis(ethyl)amino-4,12-diazahexadecane (BE-3-8-3), and 1,15-bis(ethyl)amino-4,11-diazapentadecane (BE-3-7-3) in human brain tumor cell lines U-251 MG, SF-126, and SF-188. Analog concentrations ≤ 5 μM inhibited growth and colony-forming efficiency in each cell line by treatment day 5, with significant decreases in putrescine and spermidine, but not spermine, levels. These findings suggest that potentially cytotoxic polyamine analogs can be specified on the basis of their hydrocarbon chain length and DNA affinity.
KW - Cell growth
KW - Cell survival
KW - Human brain tumor cell lines
KW - Intracellular polyamines
KW - Polyamine analogs
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M3 - Article
C2 - 8239531
AN - SCOPUS:0027484033
SN - 0250-7005
VL - 13
SP - 1525
EP - 1532
JO - Anticancer research
JF - Anticancer research
IS - 5 A
ER -