TY - JOUR
T1 - Effects of surface characteristics on non-specific agglutination in latex immunoagglutination antibody assay
AU - Yoon, Jeong Yeol
AU - Kim, Kyung Hee
AU - Choi, Sung Wook
AU - Kim, Jung Hyun
AU - Kim, Woo Sik
N1 - Funding Information:
Funding for this research was provided by the Bioproduct Research Center of Yonsei University, and the Korea Institute of S&T Evaluation and Planning (National Research Laboratory Program, 2000-N-NL-01-C-032).
PY - 2003/1/1
Y1 - 2003/1/1
N2 - To monitor the non-specific agglutination (NSA) in latex immunoagglutination assay, antigen-coated structured latex particles, which have carboxyl and sulphonate groups as hydrophilic domains, were tested for an antibody assay. Sulphonated particles showed NSA in high antibody concentrations, where no surface antigen left to match with. This was further justified with the more stable highly sulphonated particles, which showed higher degree of NSA. It can therefore be confirmed that sulphonate groups cause (or at least promote) NSA, while carboxyl groups do not. Surface coverage over 17% was not fully utilized for antigen-antibody reaction, due to the prozone effect. The difference in sensitivity of particles was explained in terms of our new explanations on the governing interactions of protein adsorption.
AB - To monitor the non-specific agglutination (NSA) in latex immunoagglutination assay, antigen-coated structured latex particles, which have carboxyl and sulphonate groups as hydrophilic domains, were tested for an antibody assay. Sulphonated particles showed NSA in high antibody concentrations, where no surface antigen left to match with. This was further justified with the more stable highly sulphonated particles, which showed higher degree of NSA. It can therefore be confirmed that sulphonate groups cause (or at least promote) NSA, while carboxyl groups do not. Surface coverage over 17% was not fully utilized for antigen-antibody reaction, due to the prozone effect. The difference in sensitivity of particles was explained in terms of our new explanations on the governing interactions of protein adsorption.
KW - Anti-BSA
KW - Anti-bovine serum albumin
KW - LAT
KW - Latex agglutination test
KW - Latex immunoagglutination assay
KW - Non-specific agglutination
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U2 - 10.1016/S0927-7765(01)00211-9
DO - 10.1016/S0927-7765(01)00211-9
M3 - Article
AN - SCOPUS:0037210378
SN - 0927-7765
VL - 27
SP - 3
EP - 9
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
IS - 1
ER -