Effects of subminimum inhibitory concentrations of antibiotics on the Pasteurella multocida proteome

Bindu Nanduri; Mark L. Lawrence; Carolyn E. Boyle; Mahalingam Ramkumar; Shane C. Burgess

doi:10.1021/pr050360r

Effects of subminimum inhibitory concentrations of antibiotics on the Pasteurella multocida proteome

Bindu Nanduri, Mark L. Lawrence, Carolyn E. Boyle, Mahalingam Ramkumar, Shane C. Burgess

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Subminimum inhibitory concentrations (sub-MICs) of antibiotics can be therapeutically effective, but the underlying molecular mechanisms are not well-characterized. We analyzed the Pasteurella multocida proteome response to sub-MICs of amoxicillin, chlortetracycline, and enrofloxacin using isotope-coded affinity tags (ICAT). There were parallel effects on inhibition of growth kinetics and suppression of protein expression by clusters of orthologous groups (COG) categories. Potential compensatory mechanisms enabling antibiotic adaptation were identified, including increased RecA expression caused by enrofloxacin.

Original language	English (US)
Pages (from-to)	572-580
Number of pages	9
Journal	Journal of Proteome Research
Volume	5
Issue number	3
DOIs	https://doi.org/10.1021/pr050360r
State	Published - Mar 2006

Keywords

Amoxicillin
Antibiotic
Bovine respiratory disease
Chlortetracycline
Clusters of orthologous groups
Enrofloxacin
ICAT
Isotope-coded affinity tag
RecA
Subminimum inhibitory concentration

ASJC Scopus subject areas

Biochemistry
Chemistry(all)

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10.1021/pr050360r

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title = "Effects of subminimum inhibitory concentrations of antibiotics on the Pasteurella multocida proteome",

abstract = "Subminimum inhibitory concentrations (sub-MICs) of antibiotics can be therapeutically effective, but the underlying molecular mechanisms are not well-characterized. We analyzed the Pasteurella multocida proteome response to sub-MICs of amoxicillin, chlortetracycline, and enrofloxacin using isotope-coded affinity tags (ICAT). There were parallel effects on inhibition of growth kinetics and suppression of protein expression by clusters of orthologous groups (COG) categories. Potential compensatory mechanisms enabling antibiotic adaptation were identified, including increased RecA expression caused by enrofloxacin.",

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AU - Nanduri, Bindu

AU - Lawrence, Mark L.

AU - Boyle, Carolyn E.

AU - Ramkumar, Mahalingam

AU - Burgess, Shane C.

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AB - Subminimum inhibitory concentrations (sub-MICs) of antibiotics can be therapeutically effective, but the underlying molecular mechanisms are not well-characterized. We analyzed the Pasteurella multocida proteome response to sub-MICs of amoxicillin, chlortetracycline, and enrofloxacin using isotope-coded affinity tags (ICAT). There were parallel effects on inhibition of growth kinetics and suppression of protein expression by clusters of orthologous groups (COG) categories. Potential compensatory mechanisms enabling antibiotic adaptation were identified, including increased RecA expression caused by enrofloxacin.

KW - Amoxicillin

KW - Antibiotic

KW - Bovine respiratory disease

KW - Chlortetracycline

KW - Clusters of orthologous groups

KW - Enrofloxacin

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Effects of subminimum inhibitory concentrations of antibiotics on the Pasteurella multocida proteome

Abstract

Keywords

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