TY - JOUR
T1 - Effects of selenium in vitro on human T-lymphocyte functions and K-562 tumor cell growth
AU - Watson, R. R.
AU - Moriguchi, S.
AU - McRae, B.
AU - Tobin, L.
AU - Mayberry, J. C.
AU - Lucas, D.
PY - 1986
Y1 - 1986
N2 - In vitro E-rosette formation, lymphocyte mitogenesis, and natural killer (NK) cell activity of human blood lymphocytes were strongly inhibited by high concentrations (10-4 M) of sodium selenite, sodium selenate, and selenium dioxide. Lower concentrations (10-5 or 10-7 M) also inhibited E-rosette formation and natural killer cell activity against K-562 tumor cells. Lymphocyte transformation induced by concanavalin A (con A) or pokeweed mitogen (PWM) was also inhibited by all selenium compounds tested, but only at the highest concentrations (10-5 and 10-4 M). There was depression of the total number of viable lymphocytes following incubation with selenium dioxide only at a high concentration (10-4 M). Interferon production was enhanced at lower levels (10-9 to 10-6 M) of selenium dioxide while a higher concentration (10-5 and 10-4 M) appeared to inhibit its production. The mechanism of inhibition by selenium compounds (10-4 M) is due, in part, to the decrease of viable lymphocytes. It is unclear how other and lower concentrations (10-7 or 10-9 M) of selenium compounds inhibit E-rosette formation, NK activity, or K-562 tumor cell growth.
AB - In vitro E-rosette formation, lymphocyte mitogenesis, and natural killer (NK) cell activity of human blood lymphocytes were strongly inhibited by high concentrations (10-4 M) of sodium selenite, sodium selenate, and selenium dioxide. Lower concentrations (10-5 or 10-7 M) also inhibited E-rosette formation and natural killer cell activity against K-562 tumor cells. Lymphocyte transformation induced by concanavalin A (con A) or pokeweed mitogen (PWM) was also inhibited by all selenium compounds tested, but only at the highest concentrations (10-5 and 10-4 M). There was depression of the total number of viable lymphocytes following incubation with selenium dioxide only at a high concentration (10-4 M). Interferon production was enhanced at lower levels (10-9 to 10-6 M) of selenium dioxide while a higher concentration (10-5 and 10-4 M) appeared to inhibit its production. The mechanism of inhibition by selenium compounds (10-4 M) is due, in part, to the decrease of viable lymphocytes. It is unclear how other and lower concentrations (10-7 or 10-9 M) of selenium compounds inhibit E-rosette formation, NK activity, or K-562 tumor cell growth.
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U2 - 10.1002/jlb.39.4.447
DO - 10.1002/jlb.39.4.447
M3 - Article
C2 - 2419467
AN - SCOPUS:0022620663
SN - 0741-5400
VL - 39
SP - 447
EP - 456
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
IS - 4
ER -