Effects of nicotine and cotinine on bovine theca intema and granulosa cells

Sara R. Sanders; S. Peder Cuneo; Adele M. Turzillo

doi:10.1016/S0890-6238(02)00049-7

Effects of nicotine and cotinine on bovine theca intema and granulosa cells

Sara R. Sanders, S. Peder Cuneo, Adele M. Turzillo

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

The purpose of this study was to determine if nicotine or cotinine inhibits steroidogenesis in the ovarian follicle. Theca interna and granulosa cells were isolated from bovine follicles, cultured with nicotine or cotinine for 24 h, and culture media were assayed for androstenedione or estradiol. Treatment of theca interna with 6, 60, and 600 μM nicotine decreased (P ≤ 0.002) production of androstenedione to 55, 53, and 24% of control levels, respectively. Levels of androstenedione in theca interna treated with cotinine were not different from control values. In granulosa cells, nicotine inhibited production of estradiol at the highest dose tested. Treatment with 600 μM nicotine decreased (P ≤ 0.001) estradiol concentration to 12% of control values, attributable to a general cytotoxic effect. Cotinine had no effect on estradiol production by granulosa cells. These results provide novel evidence for inhibitory effects of nicotine on androgen production by theca interna.

Original language	English (US)
Pages (from-to)	795-800
Number of pages	6
Journal	Reproductive Toxicology
Volume	16
Issue number	6
DOIs	https://doi.org/10.1016/S0890-6238(02)00049-7
State	Published - Nov 2002

Keywords

Androstenedione
Cotinine
Estradiol
Granulosa cells
Nicotine
Ovary
Steroidogenesis
Theca cells

ASJC Scopus subject areas

Toxicology

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10.1016/S0890-6238(02)00049-7

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title = "Effects of nicotine and cotinine on bovine theca intema and granulosa cells",

abstract = "The purpose of this study was to determine if nicotine or cotinine inhibits steroidogenesis in the ovarian follicle. Theca interna and granulosa cells were isolated from bovine follicles, cultured with nicotine or cotinine for 24 h, and culture media were assayed for androstenedione or estradiol. Treatment of theca interna with 6, 60, and 600 μM nicotine decreased (P ≤ 0.002) production of androstenedione to 55, 53, and 24% of control levels, respectively. Levels of androstenedione in theca interna treated with cotinine were not different from control values. In granulosa cells, nicotine inhibited production of estradiol at the highest dose tested. Treatment with 600 μM nicotine decreased (P ≤ 0.001) estradiol concentration to 12% of control values, attributable to a general cytotoxic effect. Cotinine had no effect on estradiol production by granulosa cells. These results provide novel evidence for inhibitory effects of nicotine on androgen production by theca interna.",

keywords = "Androstenedione, Cotinine, Estradiol, Granulosa cells, Nicotine, Ovary, Steroidogenesis, Theca cells",

author = "Sanders, {Sara R.} and Cuneo, {S. Peder} and Turzillo, {Adele M.}",

note = "Funding Information: We thank Sandra Dugas for technical assistance and the University of Arizona Dairy Research Facility for providing daily care and management of the animals used in this study. We also thank Dr. Patricia B. Hoyer for helpful comments and suggestions during preparation of this manuscript. This work was supported by a grant from the Arizona Disease Control Research Commission (Contract number 5008).",

year = "2002",

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doi = "10.1016/S0890-6238(02)00049-7",

language = "English (US)",

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AU - Sanders, Sara R.

AU - Cuneo, S. Peder

AU - Turzillo, Adele M.

N1 - Funding Information: We thank Sandra Dugas for technical assistance and the University of Arizona Dairy Research Facility for providing daily care and management of the animals used in this study. We also thank Dr. Patricia B. Hoyer for helpful comments and suggestions during preparation of this manuscript. This work was supported by a grant from the Arizona Disease Control Research Commission (Contract number 5008).

PY - 2002/11

Y1 - 2002/11

N2 - The purpose of this study was to determine if nicotine or cotinine inhibits steroidogenesis in the ovarian follicle. Theca interna and granulosa cells were isolated from bovine follicles, cultured with nicotine or cotinine for 24 h, and culture media were assayed for androstenedione or estradiol. Treatment of theca interna with 6, 60, and 600 μM nicotine decreased (P ≤ 0.002) production of androstenedione to 55, 53, and 24% of control levels, respectively. Levels of androstenedione in theca interna treated with cotinine were not different from control values. In granulosa cells, nicotine inhibited production of estradiol at the highest dose tested. Treatment with 600 μM nicotine decreased (P ≤ 0.001) estradiol concentration to 12% of control values, attributable to a general cytotoxic effect. Cotinine had no effect on estradiol production by granulosa cells. These results provide novel evidence for inhibitory effects of nicotine on androgen production by theca interna.

AB - The purpose of this study was to determine if nicotine or cotinine inhibits steroidogenesis in the ovarian follicle. Theca interna and granulosa cells were isolated from bovine follicles, cultured with nicotine or cotinine for 24 h, and culture media were assayed for androstenedione or estradiol. Treatment of theca interna with 6, 60, and 600 μM nicotine decreased (P ≤ 0.002) production of androstenedione to 55, 53, and 24% of control levels, respectively. Levels of androstenedione in theca interna treated with cotinine were not different from control values. In granulosa cells, nicotine inhibited production of estradiol at the highest dose tested. Treatment with 600 μM nicotine decreased (P ≤ 0.001) estradiol concentration to 12% of control values, attributable to a general cytotoxic effect. Cotinine had no effect on estradiol production by granulosa cells. These results provide novel evidence for inhibitory effects of nicotine on androgen production by theca interna.

KW - Androstenedione

KW - Cotinine

KW - Estradiol

KW - Granulosa cells

KW - Nicotine

KW - Ovary

KW - Steroidogenesis

KW - Theca cells

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Effects of nicotine and cotinine on bovine theca intema and granulosa cells

Abstract

Keywords

ASJC Scopus subject areas

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