Effects of ischemia and hemoglobin on vascular function in isolated rabbit aortas

Antonio J. Ruiz, Barry B.K. Chan, Todd A. Meyerhoefer, Darrell R. Buck, Patricia C. Farley, Terry L. Flanagan, Irving L. Kron

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


The vascular endothelium is important in the modulation of vascular tone via production of endothelium-derived relaxing and contracting factors. The abdominal aortas of five groups of rabbits were subjected to varying lengths of ischemia (0, 1, 2, 3, or 4 hours), removed, sectioned into transverse rings, and placed in tissue baths containing Krebs' buffer at 37°C and aerated with 95% O2/5% CO2. After equlibration the rings were tested for endothelium-dependent vasodilation with methacholine and nonendotheliumdependent vasodilation with nitroprusside. Endothelium-dependent relaxation as elicited by methacholine was impaired at 3 and 4 hours of ischemia but was not significantly different at 1 and 2 hours as compared to control, whereas endothelium-independent vasodilation remained normal throughout the different periods of ischemia. The addition of 1 × 10-6 mol/L rabbit hemoglobin reduced the time needed to demonstrate significant impairment of endothelial function to 2 hours. Endothelium-independent vasodilation was not affected by hemoglobin. We conclude that hemoglobin exacerbates ischemia vascular dysfunction in the rabbit aorta.

Original languageEnglish (US)
Pages (from-to)487-490
Number of pages4
JournalJournal of vascular surgery
Issue number4
StatePublished - Apr 1991

ASJC Scopus subject areas

  • Surgery
  • Cardiology and Cardiovascular Medicine


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