Abstract
1. 1. Effects of incubating snake and chicken kidney slices in media of varying potassium and sodium concentrations on the sodium-potassium-activated adenosine triphosphatase (Na-K-ATPase) activity of these slices were evaluated. 2. 2. Prolonged (2-hr) incubation of chicken kidney slices in potassium-free medium produced significant residual depression in Na-K-ATPase activity in heavy microsomes. Similar incubation of snake kidney slices in potassium-free medium had no residual effect on Na-K-ATPase activity. 3. 3. When potassium-depleted chicken kidney slices were incubated in the media of increasing potassium concentration, heavy microsomal specific Na-K-ATPase activity increased, but did not approach control value until medium potassium reached 40 mM. Recovery of Na-K-ATPase activity paralleled recovery of tissue potassium, suggesting the two may be related. 4. 4. Incubation of chicken slices in potassium-free medium had no residual effect on whole homogenate specific Na-K-ATPase activity, although all values were low. 5. 5. Incubation of chicken and snake kidney slices in low sodium medium had no residual effect on Na-K-ATPase activity.
Original language | English (US) |
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Pages (from-to) | 79-88 |
Number of pages | 10 |
Journal | Comparative Biochemistry and Physiology -- Part B: Biochemistry and |
Volume | 41 |
Issue number | 1 |
DOIs | |
State | Published - Jan 15 1972 |
Keywords
- Gallus domesticus
- Na-K-ATPase
- Thamnophis sp
- chicken kidney slices
- incubation media and Na-K-ATPase
- low potassium and Na-K-ATPase
- snake kidney slices
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Aquatic Science
- Animal Science and Zoology
- Molecular Biology