TY - JOUR
T1 - Effects of incubation temperature, inoculum size, and medium on agreement of macro- and microdilution broth susceptibility test results for yeasts
AU - Cook, R. A.
AU - McIntyre, K. A.
AU - Galgiani, J. N.
PY - 1990
Y1 - 1990
N2 - We examined the effects of temperature and inoculum on the agreement of macro- and microdilution broth MICs of five antifungal agents against six isolates of Candida species or Torulopsis glabrata. Incubation temperature affected results with amphotericin B, flucytosine, fluconazole, and SCH 39304, producing better agreement at 35°C than at 37°C. Agreement between methods was better with an inoculum size of 102 than with one of 104 yeast cells per ml in testing fluconazole or SCH 39304, and the discrepancies seen with a higher incubation temperature and a larger inoculum appeared to be additive. However, inoculum size did not seem to affect agreement between methods in testing amphotericin B, flucytosine, or ketoconazole, Regardless of test conditions, macrodilution broth MICs of amphotericin B for different isolates were strikingly higher than microdilution test MICs, with mean differences being greater than ninefold under some test conditions. We conclude that for most currently available antifungal agents, an incubation temperature of 35°C and a starting yeast inoculum of less than 104 cells per ml improve the agreement between macro- and microdilution broth tests.
AB - We examined the effects of temperature and inoculum on the agreement of macro- and microdilution broth MICs of five antifungal agents against six isolates of Candida species or Torulopsis glabrata. Incubation temperature affected results with amphotericin B, flucytosine, fluconazole, and SCH 39304, producing better agreement at 35°C than at 37°C. Agreement between methods was better with an inoculum size of 102 than with one of 104 yeast cells per ml in testing fluconazole or SCH 39304, and the discrepancies seen with a higher incubation temperature and a larger inoculum appeared to be additive. However, inoculum size did not seem to affect agreement between methods in testing amphotericin B, flucytosine, or ketoconazole, Regardless of test conditions, macrodilution broth MICs of amphotericin B for different isolates were strikingly higher than microdilution test MICs, with mean differences being greater than ninefold under some test conditions. We conclude that for most currently available antifungal agents, an incubation temperature of 35°C and a starting yeast inoculum of less than 104 cells per ml improve the agreement between macro- and microdilution broth tests.
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U2 - 10.1128/AAC.34.8.1542
DO - 10.1128/AAC.34.8.1542
M3 - Article
C2 - 2221863
AN - SCOPUS:0025024603
SN - 0066-4804
VL - 34
SP - 1542
EP - 1545
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
IS - 8
ER -