Effects of fetal bovine serum on ferrous ion-induced oxidative stress in pheochromocytoma (PC12) cells

Jin H. Song, Michael S. Harris, Seon H. Shin

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Ferrous ion (Fe2+) has been considered to be a cause of neuronal oxidative injury. Since body fluids contain protein and serum is an essential component of tissue culture medium, we have examined the role of serum protein on Fe2+-mediated oxidative stress using PC12 cells and rat cerebral cortices. Fe2+ or the combination of ascorbate and Fe2+ increased concentrations of thiobarbituric acid reactive substances (TBARS) in PC12 cells and cerebrocortical homogenates in medium (RPMI 1640), but did not increase TBARS when the medium was supplemented with 10% fetal bovine serum. Treatment with ascorbate/Fe2+ in serum-free medium reduced endogenous glutathione (GSH) concentration in PC12 cells. However, the medium supplemented with serum did not reduce GSH concentrations. PC12 cell death induced by ascorbate/Fe2+ was alleviated by increasing serum or bovine albumin concentrations in the medium. These observations indicated that oxidative injury caused by the transition metal ion could be lessened by adding fetal bovine serum to culture medium.

Original languageEnglish (US)
Article number342029
Pages (from-to)407-414
Number of pages8
JournalNeurochemical Research
Issue number4
StatePublished - 2001
Externally publishedYes


  • Ascorbate
  • Glutathione
  • Oxidative stress
  • Serum
  • Transition metal ion

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience


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