TY - JOUR
T1 - Effects of exogenous insulin, glucagon, and somatostatin on islet hormone secretion in the perfused chicken pancreas
AU - Honey, Richard N.
AU - Fallon, Michael B.
AU - Weir, Gordon C.
N1 - Funding Information:
From the Department of Medicine, Medical College of Virginia, Richmond, Va. Receivedfor publication March 20. 1980. Supported in part by grants from the Juvenile Diabetes Foundation and the National Institute of Arthritis, Metabolism, and Digestive Disease, Grant No AM-20349 and AM-19343. G. C. W. is a recipient of a Research Career Development Award from the National Institutes of Health, Grant No. AM-00255. Address reprint requests to G. C. Weir, Box 693, Medical College of Virginia, Richmond, Va. 23298. 0 1980 by Grune & Stratton, Inc. 002&0495/80/2912-0007$01.00/0
PY - 1980
Y1 - 1980
N2 - The effects of exogenous insulin were examined in the isolated perfused chicken pancreas with the duodenum excluded. At low background glucose (50 mg/dl), exogenous insulin infused at a concentration of 20,000 μU/ml elicited clear stimulation of somatostatin secretion while simultaneously inhibiting glucagon release. When the background glucose concentration was elevated to 750 mg/dl, exogenous insulin had no effect on either somatostatin or glucagon release. When graded doses of exogenous insulin were infused into the chicken pancreas at low background glucose, low concentrations (200 μU/ml) had little effect on somatostatin or glucagon release, but higher concentrations (2000 and 20,000 μU/ml) had clear effects on both somatostatin and glucagon secretion. Glucagon infused at 100 ng/ml stimulated both insulin and somatostatin release. When somatostatin was infused at 25 ng/ml, clear inhibition of glucagon was seen with insulin inhibited to a lesser extent. This study supports the notion of a negative feedback relation between B and D-cells of the pancreatic islets and suggests a paracrine mediation.
AB - The effects of exogenous insulin were examined in the isolated perfused chicken pancreas with the duodenum excluded. At low background glucose (50 mg/dl), exogenous insulin infused at a concentration of 20,000 μU/ml elicited clear stimulation of somatostatin secretion while simultaneously inhibiting glucagon release. When the background glucose concentration was elevated to 750 mg/dl, exogenous insulin had no effect on either somatostatin or glucagon release. When graded doses of exogenous insulin were infused into the chicken pancreas at low background glucose, low concentrations (200 μU/ml) had little effect on somatostatin or glucagon release, but higher concentrations (2000 and 20,000 μU/ml) had clear effects on both somatostatin and glucagon secretion. Glucagon infused at 100 ng/ml stimulated both insulin and somatostatin release. When somatostatin was infused at 25 ng/ml, clear inhibition of glucagon was seen with insulin inhibited to a lesser extent. This study supports the notion of a negative feedback relation between B and D-cells of the pancreatic islets and suggests a paracrine mediation.
UR - http://www.scopus.com/inward/record.url?scp=0019276901&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019276901&partnerID=8YFLogxK
U2 - 10.1016/0026-0495(80)90152-3
DO - 10.1016/0026-0495(80)90152-3
M3 - Article
C2 - 6109226
AN - SCOPUS:0019276901
SN - 0026-0495
VL - 29
SP - 1242
EP - 1246
JO - Metabolism: Clinical and Experimental
JF - Metabolism: Clinical and Experimental
IS - 12
ER -