TY - JOUR
T1 - Effects of a single intrastrand d(GpG) platinum adduct on the strand separating activity of the Escherichia coli proteins RecB and RecA
AU - Villani, Giuseppe
AU - Cazaux, Christophe
AU - Pillaire, Marie Jeanne
AU - Boehmer, Paul
N1 - Funding Information:
AcknowledgemenWtse: arem uch indebtedt o Dr. Neil P. Johnson for discussionsa nd suggestionsT.h is work was partiallys upportedb y a grantf rom the Ligue Nationalec ontrel e Cancer,c omited e la Haute-Garonne.
PY - 1993/10/25
Y1 - 1993/10/25
N2 - RecB and RecA proteins play key roles in the process ofDNA recombination in Escherichia coli and both possess DNA unwinding activities which can displace short regions of duplex DNA in an ATP-dependent manner in vitro. We have examined the effect of the most abundant DNA adduct caused by the chemotherapeutic agent cM-diamminedichloroplatinum(II) on those activities. For this purpose, we have constructed a partially duplex synthetic oligonucleotide containing the intrastrand d(GpG) crosslink positioned at a specific site. We report here that both the DNA strand separating and DNA-dependent ATPase activities of the RecB protein are inhibited by the d(GpG) cis-ddp adduct. In contrast, neither the unwinding nor the ATPase activities of RecA protein appear to be perturbed by this lesion.
AB - RecB and RecA proteins play key roles in the process ofDNA recombination in Escherichia coli and both possess DNA unwinding activities which can displace short regions of duplex DNA in an ATP-dependent manner in vitro. We have examined the effect of the most abundant DNA adduct caused by the chemotherapeutic agent cM-diamminedichloroplatinum(II) on those activities. For this purpose, we have constructed a partially duplex synthetic oligonucleotide containing the intrastrand d(GpG) crosslink positioned at a specific site. We report here that both the DNA strand separating and DNA-dependent ATPase activities of the RecB protein are inhibited by the d(GpG) cis-ddp adduct. In contrast, neither the unwinding nor the ATPase activities of RecA protein appear to be perturbed by this lesion.
KW - DNA helicase activity
KW - Escherichia coli RecA and RecB proteins
KW - cis-Diamminedichloroplatinum(II) d(GpG) adduct
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U2 - 10.1016/0014-5793(93)80380-D
DO - 10.1016/0014-5793(93)80380-D
M3 - Article
C2 - 8224177
AN - SCOPUS:0027363992
SN - 0014-5793
VL - 333
SP - 89
EP - 95
JO - FEBS Letters
JF - FEBS Letters
IS - 1-2
ER -