Effects of [1-Nα-trinitrophenylhistidine, 12-homoarginine]glucagon on cyclic AMP levels and free fatty acid release in isolated rat adipocytes

J. Adolfo García-Sáinz; Lourdes Sánchez-Sevilla; John T. Pelton; Dev Trivedi; Victor J. Hruby

doi:10.1016/0167-4889(86)90150-3

Effects of [1-N^α-trinitrophenylhistidine, 12-homoarginine]glucagon on cyclic AMP levels and free fatty acid release in isolated rat adipocytes

J. Adolfo García-Sáinz, Lourdes Sánchez-Sevilla, John T. Pelton, Dev Trivedi, Victor J. Hruby

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

[1-N^α-Trinitrophenylhistidine, 12-homoarginine]glucagon (THG) stimulated, in a concentration-dependent fashion, lipolysis (2-fold) and cyclic AMP accumulation (50% over basal) in isolated rat adipocytes, but was much less effective than glucagon, which stimulated lipolysis 4-fold and cyclic AMP accumulation 10-15-fold. THG displaced to the right the concentration-response curves for glucagon and diminished in a concentration-dependent fashion the effects of a fixed concentration of glucagon. The data indicate that THG is a mixed agonist-antagonist (partial agonist) in isolated rat fat cells.

Original language	English (US)
Pages (from-to)	310-315
Number of pages	6
Journal	BBA - Molecular Cell Research
Volume	886
Issue number	2
DOIs	https://doi.org/10.1016/0167-4889(86)90150-3
State	Published - Apr 29 1986
Externally published	Yes

Keywords

(Rat adipocyte)
Chemical modification
Glucagon agonist
Glucagon analog
Glucagon antagonist
cyclic AMP accumulation

ASJC Scopus subject areas

Molecular Biology
Cell Biology

Access to Document

10.1016/0167-4889(86)90150-3

Cite this

@article{c92b3f2bdf054daea9884c76da739393,

title = "Effects of [1-Nα-trinitrophenylhistidine, 12-homoarginine]glucagon on cyclic AMP levels and free fatty acid release in isolated rat adipocytes",

abstract = "[1-Nα-Trinitrophenylhistidine, 12-homoarginine]glucagon (THG) stimulated, in a concentration-dependent fashion, lipolysis (2-fold) and cyclic AMP accumulation (50% over basal) in isolated rat adipocytes, but was much less effective than glucagon, which stimulated lipolysis 4-fold and cyclic AMP accumulation 10-15-fold. THG displaced to the right the concentration-response curves for glucagon and diminished in a concentration-dependent fashion the effects of a fixed concentration of glucagon. The data indicate that THG is a mixed agonist-antagonist (partial agonist) in isolated rat fat cells.",

keywords = "(Rat adipocyte), Chemical modification, Glucagon agonist, Glucagon analog, Glucagon antagonist, cyclic AMP accumulation",

author = "Garc{\'i}a-S{\'a}inz, {J. Adolfo} and Lourdes S{\'a}nchez-Sevilla and Pelton, {John T.} and Dev Trivedi and Hruby, {Victor J.}",

note = "Funding Information: This work was supported in part by a Grant from CONACyT (PCSABNA 22620) and by a Grant from the U.S. Public Health Service AM21085. The authors thank Ms. Guadalupe Ramirez for skillfully typing the manuscript. V.J.H. was a 1984 Guggenheim Fellow and J.A.G.-S. in a 1985 Guggenheim Fellow.",

year = "1986",

month = apr,

day = "29",

doi = "10.1016/0167-4889(86)90150-3",

language = "English (US)",

volume = "886",

pages = "310--315",

journal = "BBA - Molecular Cell Research",

issn = "0167-4889",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Effects of [1-Nα-trinitrophenylhistidine, 12-homoarginine]glucagon on cyclic AMP levels and free fatty acid release in isolated rat adipocytes

AU - García-Sáinz, J. Adolfo

AU - Sánchez-Sevilla, Lourdes

AU - Pelton, John T.

AU - Trivedi, Dev

AU - Hruby, Victor J.

N1 - Funding Information: This work was supported in part by a Grant from CONACyT (PCSABNA 22620) and by a Grant from the U.S. Public Health Service AM21085. The authors thank Ms. Guadalupe Ramirez for skillfully typing the manuscript. V.J.H. was a 1984 Guggenheim Fellow and J.A.G.-S. in a 1985 Guggenheim Fellow.

PY - 1986/4/29

Y1 - 1986/4/29

N2 - [1-Nα-Trinitrophenylhistidine, 12-homoarginine]glucagon (THG) stimulated, in a concentration-dependent fashion, lipolysis (2-fold) and cyclic AMP accumulation (50% over basal) in isolated rat adipocytes, but was much less effective than glucagon, which stimulated lipolysis 4-fold and cyclic AMP accumulation 10-15-fold. THG displaced to the right the concentration-response curves for glucagon and diminished in a concentration-dependent fashion the effects of a fixed concentration of glucagon. The data indicate that THG is a mixed agonist-antagonist (partial agonist) in isolated rat fat cells.

AB - [1-Nα-Trinitrophenylhistidine, 12-homoarginine]glucagon (THG) stimulated, in a concentration-dependent fashion, lipolysis (2-fold) and cyclic AMP accumulation (50% over basal) in isolated rat adipocytes, but was much less effective than glucagon, which stimulated lipolysis 4-fold and cyclic AMP accumulation 10-15-fold. THG displaced to the right the concentration-response curves for glucagon and diminished in a concentration-dependent fashion the effects of a fixed concentration of glucagon. The data indicate that THG is a mixed agonist-antagonist (partial agonist) in isolated rat fat cells.

KW - (Rat adipocyte)

KW - Chemical modification

KW - Glucagon agonist

KW - Glucagon analog

KW - Glucagon antagonist

KW - cyclic AMP accumulation

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