Effect of o2 tension on the bioactivation and metabolism of aliphatic halides by primary rat-hepatocyte cultures

A. B. Direnzo; A. J. Gandolf; I. G. Sipes; K. Brendel; J. L. Byard

doi:10.3109/00498258409151441

Effect of o₂ tension on the bioactivation and metabolism of aliphatic halides by primary rat-hepatocyte cultures

A. B. Direnzo, A. J. Gandolf, I. G. Sipes, K. Brendel, J. L. Byard

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

1. The covalent binding of CCl₄ and CF₃CHBrCl to cultured rat hepatocytes was enhanced by anoxic conditions, indicative of enhanced reductive biotransformation. 2. The covalent binding of CHCl₃ and CHCl₂CH₂Cl decreased as the O₂ concentration was decreased, indicating a preference for oxidative metabolism. 3. Lower O₂ tensions decreased the formation of polar water-soluble metabolites with CHCl₃ and CHCl₂CH₂Cl, while CCl₄ and CF₃CHBrCl were unaffected. 4. The results indicate that primary cultured hepatocytes can reductively biotransform and bioactivate aliphatic halides under anoxic conditions.

Original language	English (US)
Pages (from-to)	521-525
Number of pages	5
Journal	Xenobiotica
Volume	14
Issue number	7
DOIs	https://doi.org/10.3109/00498258409151441
State	Published - 1984
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Toxicology
Pharmacology
Health, Toxicology and Mutagenesis

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title = "Effect of o2 tension on the bioactivation and metabolism of aliphatic halides by primary rat-hepatocyte cultures",

abstract = "1. The covalent binding of CCl4 and CF3CHBrCl to cultured rat hepatocytes was enhanced by anoxic conditions, indicative of enhanced reductive biotransformation. 2. The covalent binding of CHCl3 and CHCl2CH2Cl decreased as the O2 concentration was decreased, indicating a preference for oxidative metabolism. 3. Lower O2 tensions decreased the formation of polar water-soluble metabolites with CHCl3 and CHCl2CH2Cl, while CCl4 and CF3CHBrCl were unaffected. 4. The results indicate that primary cultured hepatocytes can reductively biotransform and bioactivate aliphatic halides under anoxic conditions.",

author = "Direnzo, {A. B.} and Gandolf, {A. J.} and Sipes, {I. G.} and K. Brendel and Byard, {J. L.}",

year = "1984",

doi = "10.3109/00498258409151441",

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journal = "Xenobiotica",

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TY - JOUR

T1 - Effect of o2 tension on the bioactivation and metabolism of aliphatic halides by primary rat-hepatocyte cultures

AU - Direnzo, A. B.

AU - Gandolf, A. J.

AU - Sipes, I. G.

AU - Brendel, K.

AU - Byard, J. L.

PY - 1984

Y1 - 1984

N2 - 1. The covalent binding of CCl4 and CF3CHBrCl to cultured rat hepatocytes was enhanced by anoxic conditions, indicative of enhanced reductive biotransformation. 2. The covalent binding of CHCl3 and CHCl2CH2Cl decreased as the O2 concentration was decreased, indicating a preference for oxidative metabolism. 3. Lower O2 tensions decreased the formation of polar water-soluble metabolites with CHCl3 and CHCl2CH2Cl, while CCl4 and CF3CHBrCl were unaffected. 4. The results indicate that primary cultured hepatocytes can reductively biotransform and bioactivate aliphatic halides under anoxic conditions.

AB - 1. The covalent binding of CCl4 and CF3CHBrCl to cultured rat hepatocytes was enhanced by anoxic conditions, indicative of enhanced reductive biotransformation. 2. The covalent binding of CHCl3 and CHCl2CH2Cl decreased as the O2 concentration was decreased, indicating a preference for oxidative metabolism. 3. Lower O2 tensions decreased the formation of polar water-soluble metabolites with CHCl3 and CHCl2CH2Cl, while CCl4 and CF3CHBrCl were unaffected. 4. The results indicate that primary cultured hepatocytes can reductively biotransform and bioactivate aliphatic halides under anoxic conditions.

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JO - Xenobiotica

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ER -

Effect of o₂ tension on the bioactivation and metabolism of aliphatic halides by primary rat-hepatocyte cultures

Abstract

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