Effect of mild hyperglycemia ± meta-iodo-benzylguanidine on the radiation response of R3230 AC tumors

Intae Lee; Jerry D. Glickson; Mark W. Dewhirst; Dennis B. Leeper; Randy Burd; Harish Poptani; Lydie Nadal; W. Gillies McKenna; John E. Biaglow

doi:10.1007/978-1-4615-0075-9_17

Effect of mild hyperglycemia ± meta-iodo-benzylguanidine on the radiation response of R3230 AC tumors

Intae Lee, Jerry D. Glickson, Mark W. Dewhirst, Dennis B. Leeper, Randy Burd, Harish Poptani, Lydie Nadal, W. Gillies McKenna, John E. Biaglow

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The effects of glucose or meta-iodo-benzylguanidine (MIBG) on oxygen utilization (QO₂) of several tumor cell lines were studied using a Clark-type electrode chamber. For in vivo studies, rats bearing R3230 Ac rat mammary adenocarcinomas were utilized. To evaluate changes in tumor oxygenation induced by glucose or MIBG, intratumoral pO₂ and skeletal muscle pO₂ were measured using Eppendorf Histography. To find the effect of mild hyperglycemia (i.p., 1 g/kg) ± MIBG (i.p., 20 mg/kg) on the radiation response, a growth delay assay was used. Glucose alone produced a ∼20% inhibition of QO₂ in several tumor cells we tested except Q7 tumor cells. MIBG inhibited QO₂ in R3230 Ac tumors. The median tumor pO₂ for glucose + MIBG was increased from 5.3 mm Hg to 13.8 mm Hg. We hypothesized that combined treatment with glucose + MIBG significantly enhanced radiation-induced tumoricidal effects on R3230 Ac tumors, mainly due to reduction in QO₂ and increase in tumor pO₂.

Original language	English (US)
Pages (from-to)	177-186
Number of pages	10
Journal	Advances in experimental medicine and biology
Volume	530
DOIs	https://doi.org/10.1007/978-1-4615-0075-9_17
State	Published - 2003
Externally published	Yes

Keywords

Glucose
Meta-iodo-benzylguanidine
QO
Radiation response
pO

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1007/978-1-4615-0075-9_17

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title = "Effect of mild hyperglycemia ± meta-iodo-benzylguanidine on the radiation response of R3230 AC tumors",

abstract = "The effects of glucose or meta-iodo-benzylguanidine (MIBG) on oxygen utilization (QO2) of several tumor cell lines were studied using a Clark-type electrode chamber. For in vivo studies, rats bearing R3230 Ac rat mammary adenocarcinomas were utilized. To evaluate changes in tumor oxygenation induced by glucose or MIBG, intratumoral pO2 and skeletal muscle pO2 were measured using Eppendorf Histography. To find the effect of mild hyperglycemia (i.p., 1 g/kg) ± MIBG (i.p., 20 mg/kg) on the radiation response, a growth delay assay was used. Glucose alone produced a ∼20% inhibition of QO2 in several tumor cells we tested except Q7 tumor cells. MIBG inhibited QO2 in R3230 Ac tumors. The median tumor pO2 for glucose + MIBG was increased from 5.3 mm Hg to 13.8 mm Hg. We hypothesized that combined treatment with glucose + MIBG significantly enhanced radiation-induced tumoricidal effects on R3230 Ac tumors, mainly due to reduction in QO2 and increase in tumor pO2.",

keywords = "Glucose, Meta-iodo-benzylguanidine, QO, Radiation response, pO",

author = "Intae Lee and Glickson, {Jerry D.} and Dewhirst, {Mark W.} and Leeper, {Dennis B.} and Randy Burd and Harish Poptani and Lydie Nadal and McKenna, {W. Gillies} and Biaglow, {John E.}",

year = "2003",

doi = "10.1007/978-1-4615-0075-9_17",

language = "English (US)",

volume = "530",

pages = "177--186",

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T1 - Effect of mild hyperglycemia ± meta-iodo-benzylguanidine on the radiation response of R3230 AC tumors

AU - Lee, Intae

AU - Glickson, Jerry D.

AU - Dewhirst, Mark W.

AU - Leeper, Dennis B.

AU - Burd, Randy

AU - Poptani, Harish

AU - Nadal, Lydie

AU - McKenna, W. Gillies

AU - Biaglow, John E.

PY - 2003

Y1 - 2003

N2 - The effects of glucose or meta-iodo-benzylguanidine (MIBG) on oxygen utilization (QO2) of several tumor cell lines were studied using a Clark-type electrode chamber. For in vivo studies, rats bearing R3230 Ac rat mammary adenocarcinomas were utilized. To evaluate changes in tumor oxygenation induced by glucose or MIBG, intratumoral pO2 and skeletal muscle pO2 were measured using Eppendorf Histography. To find the effect of mild hyperglycemia (i.p., 1 g/kg) ± MIBG (i.p., 20 mg/kg) on the radiation response, a growth delay assay was used. Glucose alone produced a ∼20% inhibition of QO2 in several tumor cells we tested except Q7 tumor cells. MIBG inhibited QO2 in R3230 Ac tumors. The median tumor pO2 for glucose + MIBG was increased from 5.3 mm Hg to 13.8 mm Hg. We hypothesized that combined treatment with glucose + MIBG significantly enhanced radiation-induced tumoricidal effects on R3230 Ac tumors, mainly due to reduction in QO2 and increase in tumor pO2.

AB - The effects of glucose or meta-iodo-benzylguanidine (MIBG) on oxygen utilization (QO2) of several tumor cell lines were studied using a Clark-type electrode chamber. For in vivo studies, rats bearing R3230 Ac rat mammary adenocarcinomas were utilized. To evaluate changes in tumor oxygenation induced by glucose or MIBG, intratumoral pO2 and skeletal muscle pO2 were measured using Eppendorf Histography. To find the effect of mild hyperglycemia (i.p., 1 g/kg) ± MIBG (i.p., 20 mg/kg) on the radiation response, a growth delay assay was used. Glucose alone produced a ∼20% inhibition of QO2 in several tumor cells we tested except Q7 tumor cells. MIBG inhibited QO2 in R3230 Ac tumors. The median tumor pO2 for glucose + MIBG was increased from 5.3 mm Hg to 13.8 mm Hg. We hypothesized that combined treatment with glucose + MIBG significantly enhanced radiation-induced tumoricidal effects on R3230 Ac tumors, mainly due to reduction in QO2 and increase in tumor pO2.

KW - Glucose

KW - Meta-iodo-benzylguanidine

KW - QO

KW - Radiation response

KW - pO

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ER -

Effect of mild hyperglycemia ± meta-iodo-benzylguanidine on the radiation response of R3230 AC tumors

Abstract

Keywords

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