Effect of keratin/PLGA scaffold on the articular cartilage tissue engineering: In vivo test

Su Hyun Jung, Soon Hee Kim, Jae Chan Yang, Hyun Hye Hong, Hye Lin Kim, Won Kim, Youngsook Son, Sang Jin Lee, Mark Van Dyke, James J. Yoo, John M. Rhee, Gilson Khang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Articular cartilage that is difficult to recovery when damaged needs to tissue engineering. keratin are the intermediate filament proteins that form a dense meshwork of filaments throughout the of cells and generally expressed in particular pairs of type I and type II keratin proteins in a-specific and cellular differentiation-specific manner. In this study, we are developing an alternative approach that consists of generating chondrocytes anchored to poly(L-lactide-co-glycolide)(PLGA) scaffolds impregnated keratin(keratin/PLGA) using tissue-engineering principles. We prepared PLGA and keratin/PLGA scaffolds using solvent casting/salt leaching method. Chondrocytes were isolated from the articular cartilage of New Zealand white rabbit and cultured With DMEM/Ham's F-12 supplemented with 10 % FBS, 1 % penicillin streptomycin, 200 mM L-glutamin, 50 μg/ml of ascorbic acid and 15 mM HEPES buffer 1M. After 2weeks of cell seeding, we implanted keratin /PLGA scaffolds on the back of nude mice. Morphological observation, histology, biological assay for collagen and sGAG, and PCR were performed at each time point 1, 2, 3 and 6 weeks. The cell viability and the quantity of collagen and sGAG were better keratin/PLGA scaffolds than PLGA scaffolds. Specific mRNA, type II and type I collagen, for chondrocyte expressed significantly highly in keratin/PLGA scaffold. keratin/PLGA scaffold promotes in vivo chondrocyte of rabbit articular chondrocytes. This study suggests that keratin/PLGA scaffold may serve as a potential cell delivery vehicle and a structural basis for in vivo tissue engineered articular cartilage.

Original languageEnglish (US)
Pages (from-to)192-198
Number of pages7
JournalTissue Engineering and Regenerative Medicine
Issue number1-3
StatePublished - Mar 2009
Externally publishedYes


  • Chondrocyte
  • In vivo
  • Keratin
  • PLGA
  • Scaffold

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering


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