TY - JOUR
T1 - Effect of high electrical conductivity of hydroponic nutrient solution on vaccine protein content in transgenic tomato
AU - Matsuda, Ryo
AU - Kubota, Chieri
AU - Lucrecia Alvarez, M.
AU - Cardineau, Guy A.
PY - 2012/6
Y1 - 2012/6
N2 - Using greenhouse tomato (Solanum lycopersicum) as a model system to produce pharmaceutical proteins, electrical conductivity (EC) of hydroponic nutrient solution was examined as a possible factor that affects the protein concentration in fruit. Transgenic tomato plants, expressing F1-V protein, a plantmade candidate subunit vaccine against plague (Yersinia pestis), were grown hydroponically at high (5.4 dS. m-1) or conventional EC [2.7 dS.m-1 (control)] with a high-wire system in a temperature-controlled greenhouse. There was no significant difference in plant growth and development including final shoot dry weight (DW), leaf area, stem elongation rate, or leaf development rate between high ECand control. Net photosynthetic rate, transpiration rate, and stomatal conductance (gS) of leaves were also not significantly different between EC treatments. For both EC treatments, immature green fruit accumulated DW at a similar rate, but dynamics observed in fruit total soluble protein (TSP) and F1-V during the fruit growth were different between the two ECs. Fruit TSP concentration per unit DW decreased while TSP content per whole fruit increased as fruit grew, regardless of EC. However, TSPs were significantly lower in high EC than in control. Fruit F1-V concentration per unitDWand F1-V content per whole fruit were also lower in high EC than in control. Our results found that increasing EC of nutrient solution decreased TSP including the vaccine protein in fruit, suggesting that adjusting nutrient solution EC at an appropriate level is necessary to avoid salinity stress in this transgenic tomato.
AB - Using greenhouse tomato (Solanum lycopersicum) as a model system to produce pharmaceutical proteins, electrical conductivity (EC) of hydroponic nutrient solution was examined as a possible factor that affects the protein concentration in fruit. Transgenic tomato plants, expressing F1-V protein, a plantmade candidate subunit vaccine against plague (Yersinia pestis), were grown hydroponically at high (5.4 dS. m-1) or conventional EC [2.7 dS.m-1 (control)] with a high-wire system in a temperature-controlled greenhouse. There was no significant difference in plant growth and development including final shoot dry weight (DW), leaf area, stem elongation rate, or leaf development rate between high ECand control. Net photosynthetic rate, transpiration rate, and stomatal conductance (gS) of leaves were also not significantly different between EC treatments. For both EC treatments, immature green fruit accumulated DW at a similar rate, but dynamics observed in fruit total soluble protein (TSP) and F1-V during the fruit growth were different between the two ECs. Fruit TSP concentration per unit DW decreased while TSP content per whole fruit increased as fruit grew, regardless of EC. However, TSPs were significantly lower in high EC than in control. Fruit F1-V concentration per unitDWand F1-V content per whole fruit were also lower in high EC than in control. Our results found that increasing EC of nutrient solution decreased TSP including the vaccine protein in fruit, suggesting that adjusting nutrient solution EC at an appropriate level is necessary to avoid salinity stress in this transgenic tomato.
KW - Controlled environment agriculture
KW - Molecular farming
KW - Plague
KW - Plant-made pharmaceuticals
KW - Pmp
KW - Solanum lycopersicum
KW - Subunit vaccine
UR - http://www.scopus.com/inward/record.url?scp=84863214035&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863214035&partnerID=8YFLogxK
U2 - 10.21273/horttech.22.3.362
DO - 10.21273/horttech.22.3.362
M3 - Article
AN - SCOPUS:84863214035
SN - 1063-0198
VL - 22
SP - 362
EP - 367
JO - HortTechnology
JF - HortTechnology
IS - 3
ER -