Effect of angiotensin-II on renal Na+/H+ exchanger-NHE3 and NHE2

Mehul P. Dixit, Liping Xu, Hua Xu, Liqun Bai, James F. Collins, Fayez K. Ghishan

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


The purpose of the present study was to determine the effect of angiotensin II (A-II) on membrane expression of Na+/H+ exchange isoforms NHE3 and NHE2 in the rat renal cortex. A-II (500 ng/kg per min) was chronically infused into the Sprague-Dawley rats by miniosmotic pump for 7 days. Arterial pressure and circulating plasma A-II level were significantly increased in A-II rats as compared to control rats. pH-dependent uptake of 22Na+ study in the presence of 50 μM HOE-694 revealed that Na+ uptake mediated by NHE3 was increased ∼88% in the brush border membrane from renal cortex of A-II-treated rats. Western blotting showed that A-II increased NHE3 immunoreactive protein levels in the brush border membrane of the proximal tubules by 31%. Northern blotting revealed that A-II increased NHE3 mRNA abundance in the renal cortex by 42%. A-II treatment did not alter brush border NHE2 protein abundance in the renal proximal tubules. In conclusion, chronic A-II treatment increases NHE3-mediated Na+ uptake by stimulating NHE3 mRNA and protein content.

Original languageEnglish (US)
Pages (from-to)38-44
Number of pages7
JournalBiochimica et Biophysica Acta - Biomembranes
Issue number1
StatePublished - Jul 1 2004


  • Kidney
  • Na/H exchange
  • Proximal tubule
  • Sodium uptake

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology


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