Effect of age on Agaricus bisporus PHA-B stimulated insulin release and45Ca2+ uptake in vitro by islets of langerhans

Nafees Ahmad; M. Mubin Khan; Anil K. Rastogi; Jalil R. Kidwal

doi:10.1007/BF02582088

Effect of age on Agaricus bisporus PHA-B stimulated insulin release and⁴⁵Ca²⁺ uptake in vitro by islets of langerhans

Nafees Ahmad
, M. Mubin Khan
, Anil K. Rastogi
, Jalil R. Kidwal

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The in vitro basal insulin release and⁴⁵Ca²⁺ uptake by rat islets of Langerhans remain unaffected by the animal's age. However, when Agaricus bisporus lectin PHA-B is added to the medium, there is a significant stimulation of basal insulin secretion and⁴⁵Ca²⁺ uptake. The islets isolated from younger rats are more sensitive to A. bisporus PHA-B stimulation and register 1.5-fold and 3-fold more increases of insulin release and⁴⁵Ca²⁺ uptake respectively as compared to islets isolated from 12-month-old rats. Age-related conformational changes in the B-cell membrane are suggested as a possible explanation for the above observation.

Original language	English (US)
Pages (from-to)	349-355
Number of pages	7
Journal	Acta Diabetologica Latina
Volume	21
Issue number	4
DOIs	https://doi.org/10.1007/BF02582088
State	Published - Oct 1984
Externally published	Yes

Keywords

Agaricus bisporus PHA-B
Ca
Insulin release
Islets of Langerhans

ASJC Scopus subject areas

Internal Medicine
Endocrinology, Diabetes and Metabolism
Endocrinology

Access to Document

10.1007/BF02582088

Cite this

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title = "Effect of age on Agaricus bisporus PHA-B stimulated insulin release and45Ca2+ uptake in vitro by islets of langerhans",

abstract = "The in vitro basal insulin release and45Ca2+ uptake by rat islets of Langerhans remain unaffected by the animal's age. However, when Agaricus bisporus lectin PHA-B is added to the medium, there is a significant stimulation of basal insulin secretion and45Ca2+ uptake. The islets isolated from younger rats are more sensitive to A. bisporus PHA-B stimulation and register 1.5-fold and 3-fold more increases of insulin release and45Ca2+ uptake respectively as compared to islets isolated from 12-month-old rats. Age-related conformational changes in the B-cell membrane are suggested as a possible explanation for the above observation.",

keywords = "Agaricus bisporus PHA-B, Ca, Insulin release, Islets of Langerhans",

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T1 - Effect of age on Agaricus bisporus PHA-B stimulated insulin release and45Ca2+ uptake in vitro by islets of langerhans

AU - Ahmad, Nafees

AU - Khan, M. Mubin

AU - Rastogi, Anil K.

AU - Kidwal, Jalil R.

PY - 1984/10

Y1 - 1984/10

N2 - The in vitro basal insulin release and45Ca2+ uptake by rat islets of Langerhans remain unaffected by the animal's age. However, when Agaricus bisporus lectin PHA-B is added to the medium, there is a significant stimulation of basal insulin secretion and45Ca2+ uptake. The islets isolated from younger rats are more sensitive to A. bisporus PHA-B stimulation and register 1.5-fold and 3-fold more increases of insulin release and45Ca2+ uptake respectively as compared to islets isolated from 12-month-old rats. Age-related conformational changes in the B-cell membrane are suggested as a possible explanation for the above observation.

AB - The in vitro basal insulin release and45Ca2+ uptake by rat islets of Langerhans remain unaffected by the animal's age. However, when Agaricus bisporus lectin PHA-B is added to the medium, there is a significant stimulation of basal insulin secretion and45Ca2+ uptake. The islets isolated from younger rats are more sensitive to A. bisporus PHA-B stimulation and register 1.5-fold and 3-fold more increases of insulin release and45Ca2+ uptake respectively as compared to islets isolated from 12-month-old rats. Age-related conformational changes in the B-cell membrane are suggested as a possible explanation for the above observation.

KW - Agaricus bisporus PHA-B

KW - Ca

KW - Insulin release

KW - Islets of Langerhans

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